Identification of CT521 as a frequent target of Th1 cells in patients with urogenital Chlamydia trachomatis infection
Identification of CT521 as a frequent target of Th1 cells in patients with urogenital Chlamydia trachomatis infection
复制标题
DOI：
10.1086/508203
复制
发表时间：
2006-11-01
影响因子：
6.4
通讯作者：
Theisen, Michael
中科院分区：
文献类型：
--
作者：
Olsen, Anja Weinreich;Follmann, Frank;Theisen, Michael
背景资料。为了确定新的T细胞靶点，研究了泌尿生殖道沙眼衣原体感染患者对沙眼衣原体D血清型裂解物的免疫应答。沙眼衣原体裂解物按分子质量分级，每个组分用于刺激沙眼衣原体感染患者的外周血单核细胞。在频繁识别的组分中，通过质谱学鉴定蛋白质，重组表达，并检测T细胞识别。沙眼衣原体感染患者(n=16)对这些组分的T细胞识别能力高度异质性。4名患者对16-20 kDa的抗原表现出强烈的靶向性反应。在该组分中鉴定出三种蛋白质：CT043、CT511和CT521。研究了T细胞对单个重组蛋白的反应，发现CT521诱导了最高水平的干扰素-γ。在更大的研究人群中(n=41)对CT521的识别进行了调查，83%的患者检测到了阳性的干扰素-伽马反应。在CT521中发现了多个T细胞表位，尤其是位于蛋白中央部分的多肽5经常被T细胞识别(63%)。我们已经确定了一种新的沙眼衣原体抗原，CT521，在泌尿生殖道沙眼衣原体感染的患者中经常被识别。
Background. The human immune response to a Chlamydia trachomatis serovar D lysate was investigated in patients with urogenital C. trachomatis infection, to identify novel T cell targets.Methods. A C. trachomatis lysate was fractionated on the basis of molecular mass, and each fraction was used to stimulate peripheral-blood mononuclear cells from patients with C. trachomatis infection. In frequently recognized fractions, proteins were identified by mass spectrometry, recombinantly expressed, and tested for T cell recognition.Results. T cell recognition of the fractions was highly heterogeneous in patients with C. trachomatis infection (n = 16). Four patients exhibited responses that were strongly targeted to antigens of 16-20-kDa molecular mass. Three proteins were identified in this fraction: CT043, CT511, and CT521. The T cell response to the individual recombinant proteins were investigated, and CT521 was found to induce the highest level of interferon (IFN)-gamma. The recognition of CT521 was investigated in a larger study population (n = 41), and a positive IFN-gamma response was measured in 83% of the patients. Several T cell epitopes were identified in CT521; in particular, peptide 5 in the central part of the protein was frequently recognized by T cells (63%).Conclusion. We have identified a novel C. trachomatis antigen, CT521, that is frequently recognized in patients with urogenital C. trachomatis infection.