Aparasitemic serological suspects in Trypanosoma brucei gambiense human African trypanosomiasis:: A potential human reservoir of parasites?
Aparasitemic serological suspects in Trypanosoma brucei gambiense human African trypanosomiasis:: A potential human reservoir of parasites?
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DOI：
10.1016/j.actatropica.2006.04.001
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发表时间：
2006-05-01
期刊：
影响因子：
2.7
通讯作者：
Jamonneau, V.
中科院分区：
文献类型：
--
作者：
Koffi, M.;Solano, P.;Jamonneau, V.
用于诊断人类非洲布氏锥虫(HAT)的血清学和寄生虫学试验分别具有低的特异性和敏感性，在现场，控制计划小组面临的是血清学阳性但寄生虫学阴性的受试者，他们仍然没有得到治疗。这项工作的目的是利用聚合酶链式反应工具，探索这些寄生虫病血清学嫌疑人的意义。由于不同的提取方法在早期观察到了不一致的PCR结果，所以比较了两种DNA提取方法(CHILEX 100((R))树脂和DNasy((R))组织试剂盒)。这项研究对604份血液样本进行了研究：574份血样来自寄生虫学确诊患者、寄生虫病血清学嫌疑人和地方病对照，30份血样来自法国收集的健康志愿者。两种提取方法所得结果无显著差异。关于聚合酶链式反应，观察到重复性问题，以及与血清学和寄生虫学检测结果不一致的问题，主要是针对寄生虫性阴囊学嫌疑人。除了先前的结果表明存在无毒或非致病的锥虫菌株以及个体易感性导致长期血清阳性而没有检测到寄生虫血症但聚合酶链式反应阳性外，这项研究的结果支持长期人类宿主的概念，这可能有助于地方性疫区HAT的维持或周期性复发。(C)2006爱思唯尔B.V.保留所有权利。
The serological and parasitological tests used for Trypanosoma brucei gambiense human African trypanosomiasis (HAT) diagnosis have low specificity and sensitivity, respectively, and in the field, control program teams are faced with subjects with positive serology but negative parasitology who remain untreated. The aim of this work was to explore, using PCR tool, the significance of these aparasitemic serological suspects. Since discordant PCR results have been observed earlier with different extraction methods, two DNA extraction methods were compared (the Chelex 100((R)) resin and the DNeasy((R)) Tissue kit). The study was conducted on 604 blood samples: 574 from parasitologically confirmed patients, aparasitemic serological suspects and endemic controls collected in Me d'lvoire and 30 from healthy volunteers collected in France. No significant differences were observed between the PCR results obtained with the two extraction methods. Concerning PCR, problems of reproducibility and discordances with both serological and parasitological test results were observed, mainly for the aparasitemic scrological suspects. In addition to previous results that pointed to the existence of non-virulent or non-pathogenic trypanosome strains and of individual susceptibility leading to long term seropositivity without detectable parasitaemia but positive PCR, the results of this study support the notion of a long lasting human reservoir that may contribute to the maintenance or periodic resurgences of HAT in endemic foci. (c) 2006 Elsevier B.V. All rights reserved.