Effects of Fe, salicylate and Zn on metallothionein and lipid peroxidation in vivo.
Effects of Fe, salicylate and Zn on metallothionein and lipid peroxidation in vivo.
复制标题
铁、水杨酸盐和锌对体内金属硫蛋白和脂质过氧化的影响。
DOI：
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发表时间：
1991
期刊：
影响因子：
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通讯作者：
J. Vormann
中科院分区：
文献类型：
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作者：
T. Günther;R. Grossrau;V. Höllriegl;J. Vormann
对照组大鼠和两次腹腔注射15 mg/kg Fe（以FeCl 3形式给药）预处理的大鼠接受一次口服剂量的700 mg/kg水杨酸（以水杨酸钠形式给药）或100 μ mol ZnCl 2/kg s.c.，或水杨酸盐和ZnCl 2。在给予Fe的大鼠中，这种治疗与第二次铁注射同时进行。水杨酸盐和铁单独引起的脂质过氧化（LPO）的一个小的增加，通过硫代巴比妥酸盐方法测量的肝脏中丙二醛的形成。然而，当一起给药时，铁和水杨酸盐引起肝脏中LPO的急剧增加，同时注射ZnCl 2可降低肝脏中LPO的水平。通过脂质储存法和LPO组织化学法测定肝毒性。锌对LPO和肝毒性的保护作用与金属硫蛋白的诱导无关。锌的保护作用可以解释为铁与锌竞争结合水杨酸，减少铁水杨酸诱导的LPO和肝毒性。
Control rats and rats pretreated with two i.p. injections of 15 mg/kg Fe, given as FeCl3, received either one oral dose of 700 mg/kg salicylic acid, given as Na salicylate, or 100 mumol ZnCl2/kg s.c., or salicylate and ZnCl2. In the rats given Fe this treatment was simultaneous with the second iron injection. Salicylate and Fe alone caused a small increase in lipid peroxidation (LPO) measured as malondialdehyde formation in the liver by means of the thiobarbiturate method. When given together, however, Fe and salicylate caused a drastic increase of LPO in liver, which was reduced by the simultaneous injection of ZnCl2. Hepatotoxicity as determined histochemically by lipid storage paralleled LPO. The protective effect of Zn on LPO and hepatotoxicity was not correlated to metallothionein induction in liver. The protective effect of Zn can be explained by the competition of Fe with Zn for binding to salicylate which reduces Fe-salicylate-induced LPO and hepatotoxicity.