Anti-inflammatory effect of conditioned medium from human uterine cervical stem cells in uveitis
Anti-inflammatory effect of conditioned medium from human uterine cervical stem cells in uveitis
复制标题
DOI：
10.1016/j.exer.2016.06.022
复制
发表时间：
2016-08-01
影响因子：
3.4
通讯作者：
Perez-Fernandez, Roman
中科院分区：
文献类型：
--
作者：
Bermudez, Maria A.;Sendon-Lago, Juan;Perez-Fernandez, Roman
本研究旨在评价人宫颈干细胞条件培养液(CM-hUCESCs)对葡萄膜炎的治疗作用。为此，在足垫注射大肠杆菌脂多糖(LPS)后，诱导大鼠葡萄膜炎。用脂多糖刺激的人视网膜色素上皮(ARPE-19)细胞检测CM-hUCESCs的体外抗炎作用。实时定量聚合酶链式反应检测促炎症细胞因子IL-6、IL-8、巨噬细胞炎性蛋白-1α、肿瘤坏死因子-α和抗炎因子-10的mRNA表达水平。在Neubauer小室中对房水中的白细胞(AQH)进行定量，并用苏木精-伊红染色进行眼组织病理学分析。此外，使用人细胞因子抗体阵列，我们评估了CM-hUCESCs以确定中介蛋白。结果显示，CM-hUCESCs在体外和体内均显著降低了脂多糖诱导的促炎细胞因子，并降低了AQH和眼组织中的白细胞。在CM-hUCESCs中发现了高水平的具有抗炎作用的细胞因子，提示这些因素可能在减轻眼内炎症中发挥作用。综上所述，使用CM-hUCESCs治疗可显著减少葡萄膜炎的炎症。结果表明，CM-hUCESCs可作为治疗眼部炎症的潜在药物。(C)2016爱思唯尔有限公司。保留所有权利。
The aim of the present study was to evaluate the effect of conditioned medium from human uterine cervical stem cells (CM-hUCESCs) in uveitis. To do that, uveitis was induced in rats after footpad injection of Escherichia coli lipopolysaccaride (LPS). Human retinal pigment epithelial (ARPE-19) cells after LPS challenge were used to test anti-inflammatory effect of CM-hUCESCs 'in vitro'. Real-time PCR was used to evaluate mRNA expression levels of the pro-inflammatory cytokines interkeukin-6, interkeukin-8, macrophage inflammatory protein-1 alpha, tumor necrosis factor alpha, and the anti-inflammatory interkeukin-10. Leucocytes from aqueous humor (AqH) were quantified in a Neubauer chamber, and eye histopathological analysis was done with hematoxylin-eosin staining. Additionally, using a human cytokine antibody array we evaluated CM-hUCESCs to determine mediating proteins. Results showed that administration of CM-hUCESCs significantly reduced LPS-induced pro-inflammatory cytokines both 'in vitro' and 'in vivo', and decreased leucocytes in AqH and ocular tissues. High levels of cytokines with anti-inflammatory effects were found in CM-hUCESCs, suggesting a possible role of these factors in reducing intraocular inflammation. In summary, treatment with CM-hUCESCs significantly reduces inflammation in uveitis. Our data indicate that CM-hUCESCs could be regarded as a potential therapeutic agent for patients suffering from ocular inflammation. (C) 2016 Elsevier Ltd. All rights reserved.