RHEOLOGICAL PROPERTIES OF ROSETTES FORMED BY RED-BLOOD-CELLS PARASITIZED BY PLASMODIUM-FALCIPARUM
RHEOLOGICAL PROPERTIES OF ROSETTES FORMED BY RED-BLOOD-CELLS PARASITIZED BY PLASMODIUM-FALCIPARUM
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DOI：
10.1111/j.1365-2141.1992.tb06955.x
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发表时间：
1992-12-01
影响因子：
6.5
通讯作者：
WAHLGREN, M
中科院分区：
文献类型：
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作者：
NASH, GB;COOKE, BM;WAHLGREN, M
一部分被恶性疟原虫寄生的红细胞与未被寄生的红细胞形成玫瑰花状。虽然这些玫瑰花被认为会损害微循环流动，但它们的流变特性尚未得到充分的描述。使用双微管操作分离单个玫瑰花，我们发现将玫瑰花结合在一起的力很强（去除细胞的平均力为4.4 x 10(-10) N，大约是分离粘附在培养内皮上的寄生细胞所需力的5倍）。如果被破坏的莲座重新形成，细胞在接触时立即形成莲座，但附着的强度在几分钟内增加，并且在几小时内没有明显达到最大水平。所有未被寄生的细胞都能粘附到形成玫瑰花的被寄生细胞上。莲座可以承受动脉血流压力（1.4-1.6 Pa）数分钟而不崩解。为了测试莲座对流动阻力的影响，测量了进入4.3 mm移液管所需的时间。进入时间很大程度上取决于莲座中的细胞数量，平均时间是未寄生细胞的35倍。我们的研究表明，玫瑰花体内的细胞-细胞附着物很强，这表明玫瑰花可能在动脉循环和微血管通道中存活，并可能导致恶性疟疾的缺血性并发症。
A proportion of red blood cells parasitized by Plasmodium falciparum form rosettes with non-parasitized red cells. Although these rosettes are thought to impair microcirculatory flow, their rheological characteristics have not been fully described. Using dual-micropipette manipulation to pull apart individual rosettes, we found that the forces binding rosettes together were strong (average force for removal of a cell was 4.4 x 10(-10) N, approximately 5 times that required to detach a parasitized cell adhered to cultured endothelium). If disrupted rosettes were re-formed, cells rosetted immediately on contact, but the strength of attachment increased over minutes, and did not apparently reach its maximal level for hours. All non-parasitized cells tested could adhere to rosette-forming parasitized cells. Rosettes could withstand arterial flow stresses (1.4-1.6 Pa) for minutes without disintegration. To test the effects of rosetting on flow resistance, the time required for entry into a 4.3 mum pipette was measured. Entry times depended strongly on the number of cells in the rosette, and averaged 35 times longer than for non-parasitized cells. Our studies show that the cell-cell attachments within rosettes are strong, and suggest that rosettes might survive both the arterial circulation and passage through microvessels and could contribute to the ischaemic complications of falciparum malaria.