Silk-Hydroxyapatite Nanoscale Scaffolds with Programmable Growth Factor Delivery for Bone Repair
Silk-Hydroxyapatite Nanoscale Scaffolds with Programmable Growth Factor Delivery for Bone Repair
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DOI：
10.1021/acsami.6b08180
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发表时间：
2016-09-21
影响因子：
9.5
通讯作者：
Kaplan, David L.
中科院分区：
文献类型：
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作者：
Ding, Zhaozhao;Fan, Zhihai;Kaplan, David L.
骨诱导生物材料对于修复各种骨缺损具有吸引力，而仿生策略对于开发具有这种能力的骨支架非常有用。本研究采用多重仿生设计，以提高羟基磷灰石纳米颗粒（HA）和丝素蛋白（SF）组成的复合支架的成骨能力。将SF纳米纤维与水分散性HA纳米粒子共混，制备HA均匀分布的纳米复合支架。具有高HA含量（40%），模仿骨的细胞外基质（ECM）。将骨形态发生蛋白-2（BMP-2）负载于SF和HA中以调节BMP-2的释放。体外研究表明，复合支架中BMP-2的生物活性得到了保持，并通过调节BMP-2在SF和HA上的负载比例实现了可编程的缓释。体外和体内成骨研究表明，复合支架显示。在合适的BMP-2释放条件下，改善的成骨能力明显优于先前报道的负载BMP-2的SF HA复合支架。因此，这些具有可调BMP-2递送的生物力学SF HA纳米级支架为骨再生提供了优选的微生物活性。
Osteoinductive biornaterials are attractive for repairing a variety of bone defects, and biomimetic strategies are useful toward developing bone scaffolds with such capacity. Here, a multiple biomimetic design was developed to improve the osteogenesis capacity,of composite scaffolds consisting of hydroxyapatite nanoparticies (HA) and silk fibroin (SF). SF nanofibers and water-dispersible HA nanoparticles were blended to prepare the nanoscaled composite scaffolds with a uniform distribution of HA. with a high HA content (40%), imitating the extracellular matrix (ECM) of bone. Bone morphogenetic protein-2 (BMP-2) Was loaded in the SF scaffolds and HA to tune BMP-2 release In vitro studies showed the preservation of BMP-2 bioactivity in the composite scaffolds, and programmable sustained release was achieved through adjusting the ratio of BMP-2 loaded on SF and HA. In vitro and in vivo osteogenesia studies demonstrated that the composite scaffolds showed. improved Osteogenesis, capacity under suitable BMP-2 release conditions, significantly better than that of BMP-2 loaded SF HA composite scaffolds reported previously. Therefore, these biominietic SF HA nanoscaled Scaffolds with tunable BMP-2 delivery provide preferable microenvironrnents for bone regeneration.