Differential messenger RNA distribution of lactate dehydrogenase LDH-1 and LDH-5 isoforms in the rat brain
Differential messenger RNA distribution of lactate dehydrogenase LDH-1 and LDH-5 isoforms in the rat brain
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DOI：
10.1016/s0306-4522(99)00580-1
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发表时间：
2000-01-01
期刊：
影响因子：
3.3
通讯作者：
Bouras, C
中科院分区：
文献类型：
--
作者：
Laughton, JD;Charnay, Y;Bouras, C
乳酸在脑能量代谢中的作用最近重新受到关注。尽管血源性单羧酸盐（例如乳酸）很难穿过成人大脑中的血脑屏障，但脑实质内产生的乳酸可能是脑细胞的合适底物。乳酸脱氢酶对于乳酸的生产和利用至关重要。在本文中，我们使用特定的 [α-S-35]dATP 3' 末端标记寡核苷酸探针进行原位杂交组织化学，报告了成年大鼠大脑中乳酸脱氢酶亚型 1 和 5 的信使 RNA 的区域分布。放射自显影照片显示，乳酸脱氢酶-1信使RNA在多种脑结构中高表达，包括主嗅球、梨状皮层、几个丘脑和下丘脑核、脑桥核、腹侧耳蜗核、三叉神经和孤束核。此外，小脑的颗粒层和浦肯野细胞层显示出强烈的标记。新皮质（例如扣带皮质、压后皮质和额顶叶皮质）通常表现出乳酸脱氢酶 1 信使 RNA 的明显层状分布模式（II/III、IV 和 VI 层标记最强）。相比之下，乳酸脱氢酶 5 信使 RNA 的表达通常似乎更分散地分布在不同的大脑区域。在海马结构（特别是在阿蒙角和齿状回）和大脑皮层中表达特别强烈，但没有观察到层状分布模式。总体而言，这些数据与乳酸是脑细胞产生和消耗的重要能量底物这一新兴观点是一致的。 (C) 2000 国际广播组织。由爱思唯尔科学有限公司出版
The role of lactate in brain energy metabolism has recently received renewed attention. Although blood-borne monocarboxylates such as lactate poorly cross the blood-brain barrier in the adult brain, lactate produced within the brain parenchyma may be a suitable substrate for brain cells. Lactate dehydrogenase is crucial for both the production and utilization of lactate. In this article, we report the regional distribution of the messenger RNAs for lactate dehydrogenase isoforms 1 and 5 in the adult rat brain using in situ hybridization histochemistry with specific [alpha-S-35]dATP 3' end-labeled oligoprobes. The autoradiographs revealed that the lactate dehydrogenase-1 messenger RNA is highly expressed in a variety of brain structures, including the main olfactory bulb, the piriform cortex, several thalamic and hypothalamic nuclei, the pontine nuclei, the ventral cochlear nucleus, the trigeminal nerve and the solitary tractus nucleus. In addition, the granular and Purkinje cell layers of the cerebellum showed a strong labeling. The neocortex (e.g., cingular, retrosplenial and frontoparietal cortices) often exhibits a marked laminar pattern of distribution of lactate dehydrogenase-1 messenger RNA (layers II/III, IV and VI being most strongly labeled). In contrast, expression of the lactate dehydrogenase-5 messenger RNA generally seemed more diffusely distributed across the different brain regions. Expression was particularly strong in the hippocampal formation (especially in Ammon's horn and dentate gyrus) and in the cerebral cortex, where no laminar pattern of distribution was observed.Overall, these data are consistent with the emerging idea that lactate is an important energy substrate produced and consumed by brain cells. (C) 2000 IBRO. Published by Elsevier Science Ltd.