Bone marrow cells adopt the phenotype of other cells by spontaneous cell fusion
Bone marrow cells adopt the phenotype of other cells by spontaneous cell fusion
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DOI：
10.1038/nature730
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发表时间：
2002-04-04
期刊：
影响因子：
64.8
通讯作者：
Scott, EW
中科院分区：
文献类型：
--
作者：
Terada, N;Hamazaki, T;Scott, EW
最近的研究表明，移植的骨髓细胞可以变成意想不到的谱系，包括肌细胞，肝细胞，神经元和许多其他细胞（1）。然而，一个潜在的问题是，在体内讨论这种“转分化”的报告往往根据供体特异性基因（如Y染色体标记）的存在来推断转分化细胞的供体来源（1）。在这里，我们证明了小鼠骨髓细胞可以在含有白细胞介素-3的体外培养中与胚胎干细胞自发融合。此外，自发融合的骨髓细胞随后可以采用受体细胞的表型，这在没有详细的遗传分析的情况下可能被解释为“去分化”或转分化。
Recent studies have demonstrated that transplanted bone marrow cells can turn into unexpected lineages including myocytes, hepatocytes, neurons and many others(1). A potential problem, however, is that reports discussing such 'transdifferentiation' in vivo tend to conclude donor origin of transdifferentiated cells on the basis of the existence of donor-specific genes such as Y-chromosome markers(1). Here we demonstrate that mouse bone marrow cells can fuse spontaneously with embryonic stem cells in culture in vitro that contains interleukin-3. Moreover, spontaneously fused bone marrow cells can subsequently adopt the phenotype of the recipient cells, which, without detailed genetic analysis, might be interpreted as 'dedifferentiation' or transdifferentiation.