Pollen-inspired enzymatic microparticles to reduce organophosphate toxicity in managed pollinators
Pollen-inspired enzymatic microparticles to reduce organophosphate toxicity in managed pollinators
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DOI：
10.1038/s43016-021-00282-0
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发表时间：
2021-05-20
期刊：
影响因子：
23.2
通讯作者：
Ma, Minglin
中科院分区：
文献类型：
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作者：
Chen, Jing;Webb, James;Ma, Minglin
传粉者支持世界各地主要粮食作物的生产。有机磷是一种广泛使用的杀虫剂，授粉者可能会接触到，特别是在作物授粉期间。暴露于致死或亚致死剂量会损害野生和管理蜜蜂的健康，危及授粉质量和粮食安全。在这里，我们报告了一种低成本，可扩展的有机磷杀虫剂体内解毒策略，涉及磷酸三酯酶（OPT）在花粉启发的微粒（PIM）的封装。我们开发了能够以90%的效率装载OPT并保护OPT免受蜂肠pH值降解的均匀和可消耗的PIM。喂食马拉硫磷污染的花粉饼的熊蜂小菌落在喂食OPT-PIM时表现出100%的存活率，但在五天和四天内分别用OPT单独或用纯蔗糖存活率为0%。因此，当蜜蜂食用OPT-PIM时，马拉硫磷的有害影响被消除。该设计提供了一种通用的处理方法，可以将其整合到用于管理传粉昆虫的补充饲料中，例如花粉饼或膳食糖浆，以降低有机磷杀虫剂的风险。大黄蜂喂养的花粉饼污染的磷酸三酯酶杀虫剂封装在花粉启发的微粒表现出100%的生存。
Pollinators support the production of the leading food crops worldwide. Organophosphates are a heavily used group of insecticides that pollinators can be exposed to, especially during crop pollination. Exposure to lethal or sublethal doses can impair fitness of wild and managed bees, risking pollination quality and food security. Here we report a low-cost, scalable in vivo detoxification strategy for organophosphate insecticides involving encapsulation of phosphotriesterase (OPT) in pollen-inspired microparticles (PIMs). We developed uniform and consumable PIMs capable of loading OPT at 90% efficiency and protecting OPT from degradation in the pH of a bee gut. Microcolonies of Bombus impatiens fed malathion-contaminated pollen patties demonstrated 100% survival when fed OPT-PIMs but 0% survival with OPT alone, or with plain sucrose within five and four days, respectively. Thus, the detrimental effects of malathion were eliminated when bees consumed OPT-PIMs. This design presents a versatile treatment that can be integrated into supplemental feeds such as pollen patties or dietary syrup for managed pollinators to reduce risk of organophosphate insecticides.Organophosphate insecticides are highly toxic to pollinators. Bumblebees fed pollen patties contaminated with phosphotriesterase insecticide encapsulated in pollen-inspired microparticles demonstrated 100% survival.