Transcriptome analysis of human peri-implant soft tissue and periodontal gingiva: a paired design study
Transcriptome analysis of human peri-implant soft tissue and periodontal gingiva: a paired design study
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DOI：
10.1007/s00784-023-05017-y
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发表时间：
2023-04-15
影响因子：
3.4
通讯作者：
Huang, Baoxin
中科院分区：
文献类型：
--
作者：
Chen, Danying;Li, Zhixin;Huang, Baoxin
目的：目前关于种植体周围软组织在转录水平上的生物学特性的信息有限。本研究的目的是探讨牙种植体对体内软组织的影响，通过使用配对的样品，并在转录水平上比较种植体周围的软组织和牙周gingiva. MethodsPairsperipheral软组织和牙周gingiva. Methodsperipheral软组织和牙周gingiva.6例患者，并通过深度测序的汇总RNA进行分析。应用文氏图进一步筛选出每对样品中差异表达的基因。进行注释和富集分析。结果共发现3549个差异表达基因（DEGs），其中牙周组和种植体组差异表达基因最多，差异表达基因最少。Venn图进一步鉴定了每对样品中的185个DEG，其中富集分析鉴定了细胞组分的显著富集与质膜外侧相关，分子功能是蛋白结合，生物过程是免疫系统过程，KEGG途径是精氨酸-细胞因子受体相互作用。在DEG，CST 1，SPP 1，AQP 9，和SFRP 2被验证为在种植体周围的软组织中上调。ConclusionsPeri-implant软组织显示改变了与细胞-ECM相互作用的几个基因的表达相比，牙周牙龈。
ObjectivesLimited information is available about the biological characterization of peri-implant soft tissue at the transcriptional level. The aim of this study was to investigate the effect of dental implant on the soft tissue in vivo by using paired samples and compare the differences between peri-implant soft tissue and periodontal gingiva at the transcriptional level.MethodsPaired peri-implant soft tissue and periodontal gingiva tissue from 6 patients were obtained, and the pooled RNAs were analyzed by deep sequencing. Venn diagram was used to further screen out differentially expressed genes in every pair of samples. Annotation and enrichment analysis was performed. Further verification was done by quantitative real-time PCR.ResultsTotally 3549 differentially expressed genes (DEGs) were found between peri-implant and periodontal groups. The Venn diagram further identified 185 DEGs in every pair of samples, of which the enrichment analysis identified significant enrichment for cellular component was associated with external side of plasma membrane, for molecular function was protein binding, for biological process was immune system process, and for KEGG pathway was cytokine-cytokine receptor interaction. Among the DEGs, CST1, SPP1, AQP9, and SFRP2 were verified to be upregulated in peri-implant soft tissue.ConclusionsPeri-implant soft tissue showed altered expressions of several genes related to the cell-ECM interaction compared to periodontal gingiva.