Statin, a nonproliferation-specific protein, is associated with the nuclear envelope and is heterogeneously distributed in cells leaving quiescent state.
Statin, a nonproliferation-specific protein, is associated with the nuclear envelope and is heterogeneously distributed in cells leaving quiescent state.
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他汀类药物是一种非增殖特异性蛋白，与核膜相关，并且在处于静止状态的细胞中异质分布。
DOI：
10.1002/jcp.1041400303
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发表时间：
1989
影响因子：
5.6
通讯作者：
Wang,E
中科院分区：
文献类型：
--
作者：
Wang,E
他汀是一种57,000道尔顿的蛋白质，主要存在于终末分化组织或体外老化成纤维细胞培养的非增殖细胞的细胞核中。在年轻的生长细胞中，一旦细胞周期穿越被各种组织培养操作（如血清饥饿）阻断，该蛋白可以被诱导出现在细胞核中；然而，这种表达可以通过添加血清迅速去除。他汀类药物在离开静止状态的细胞中沿细胞核周围的消失并不均匀；可呈帽状、结节状、斑块状或不规则颗粒状分布。这种不寻常的分布似乎表明，在他汀类药物残留的核膜区域存在优先位点。在离体大鼠肝核周围的荧光抗体强染色证实了氧化石墨烯静止期细胞核膜区域的他汀类药物浓度。对离体细胞核的进一步检查表明，该蛋白与核膜的层间室有关；免疫印迹实验结果显示，他汀类药物存在于层粘连蛋白A‐C富集的部分中，证明了这一点。免疫金标记研究表明，该蛋白位于核膜的一般区域。这些结果表明，他汀类药物在氧化石墨烯静止细胞中主要位于核膜区域，并且在这一区域附近可能存在他汀类药物浓度的几何位置，这可以从那些经历脱离静止状态的细胞中的不均匀分布中得到证明。
Statin, a protein of 57,000 daltons, is present primarily in the nuclei of nonproliferating cells of terminally differentiated tissues or of in vitro aged fibroblast cultures. In young growing cells, the protein can be induced to appear in the nuclei once the cell‐cycle traverse is blocked by various tissue culture manipulations, such as serum starvation; this expression, however, can be rapidly removed by addition of serum. The disappearance of statin in cells leaving the quiescent state is not uniform along the periphery of the nucleus; it can be distributed in various patterns, such as caps, nodules, patches, or irregular granules. This unusual distribution seems to suggest that preferential sites exist at the region of the nuclear envelope where statin presence may residually remain. The concentration of statin at the nuclear envelope region in cells at GO‐quiescent phase is confirmed by the intense staining of fluorescent antibody at the periphery of isolated rat liver nuclei. Further examination of the isolated nuclei reveals that the protein is associated with the lamina compartment of the nuclear envelope; this is evidenced by the results of immunoblotting experiments showing statin presence in the fraction enriched for lamins A‐C. Immunogold labelling studies show that the protein is located in the general area of the nuclear envelope. These results suggest that statin in GO‐quiescent cells is located predominantly at the nuclear envelope region and that in this vicinity there may exist geometrically sites of statin concentration as evidenced by the heterogeneous distribution in those cells experiencing the departure from the quiescent state.