Protein Condensation at Nanopore Entrances as Studied by Differential Scanning Calorimetry and Small-Angle Neutron Scattering
Protein Condensation at Nanopore Entrances as Studied by Differential Scanning Calorimetry and Small-Angle Neutron Scattering
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DOI：
10.1021/acs.jpclett.2c01708
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发表时间：
2022-09-12
影响因子：
5.7
通讯作者：
Yamaguchi，Akira
中科院分区：
文献类型：
--
作者：
Aizawa，Mami;Iwase，Hiroki;Yamaguchi，Akira
采用差示扫描量热法（DSC）和对比度匹配小角中子散射（CM-SANS）实验研究了球形肌红蛋白（Mb）在一系列孔径（4.2，6.4，7.7和9.0 nm）的介孔氧化硅（MPS）孔入口处的凝聚。进行DSC测量以估计Mb在两个不同吸附位点（即，孔内部和孔入口区域）处吸附的量。进行CM-SANS测量以观察Mb分子在孔入口区域的凝聚。值得注意的是，发现直径接近Mb直径两倍的纳米孔入口是促进球状Mb冷凝的特定空腔。在从浓缩Mb溶液的吸附摄取过程中，Mb在6.4 nm孔的入口处发生冷凝，而从稀释Mb溶液的吸附摄取诱导Mb在7.7 nm孔的入口处冷凝。
The condensation of globular myoglobin (Mb) at the pore entrances of mesoporous silica (MPS) with a series of pore diameters (4.2, 6.4, 7.7, and 9.0 nm) was examined by differential scanning calorimetry (DSC) and contrast-matching small-angle neutron scattering (CM-SANS) experiments. The DSC measurements were performed to estimate the amount of Mb adsorbed at two different adsorption sites, namely, the pore interior and the pore entrance regions. The CM-SANS measurements were conducted to observe condensation of Mb molecules at the pore entrance regions. Notably, the nanopore entrance with a diameter close to twice that of the Mb diameter was found to be the specific cavity to facilitate the condensation of globular Mb. The Mb condensation occurred at the entrances of the 6.4 nm pore during the adsorption uptake from concentrated Mb solutions, whereas the adsorption uptake from diluted Mb solutions induced the condensation of Mb at the entrances of the 7.7 nm pore.