Imaging the glycome
Imaging the glycome
复制标题
DOI：
10.1073/pnas.0811481106
复制
发表时间：
2009-01-06
影响因子：
11.1
通讯作者：
Bertozzi, Carolyn R.
中科院分区：
文献类型：
--
作者：
Laughlin, Scott T.;Bertozzi, Carolyn R.
分子成像使得能够在体内可视化特定分子，而不会对靶分子的环境产生实质性干扰。聚糖是分子成像的有吸引力的目标，但无法用常规方法获得。用于监测聚糖的经典方法依赖于具有探针的凝集素或抗体的分子识别，但是这些技术不太适合于体内成像。在一种新兴的策略中，聚糖通过用化学报告分子进行代谢标记并随后连接到荧光探针来成像。该技术使得活细胞和活生物体（如斑马鱼）中的聚糖可视化成为可能。化学报告分子成像为探索伴随发育和疾病的糖组变化提供了新的途径。
Molecular imaging enables visualization of specific molecules in vivo and without substantial perturbation to the target molecule's environment. Glycans are appealing targets for molecular imaging but are inaccessible with conventional approaches. Classic methods for monitoring glycans rely on molecular recognition with probe-bearing lectins or antibodies, but these techniques are not well suited to in vivo imaging. In an emerging strategy, glycans are imaged by metabolic labeling with chemical reporters and subsequent ligation to fluorescent probes. This technique has enabled visualization of glycans in living cells and in live organisms such as zebrafish. Molecular imaging with chemical reporters offers a new avenue for probing changes in the glycome that accompany development and disease.