An improved flow microfluorometer for rapid measurement of cell fluorescence.
An improved flow microfluorometer for rapid measurement of cell fluorescence.
复制标题
一种改进的流动微荧光计，用于快速测量细胞荧光。
DOI：
10.1016/0014-4827(73)90280-2
复制
发表时间：
1973
影响因子：
3.7
通讯作者：
L. Cram
中科院分区：
文献类型：
--
作者：
D. Holm;L. Cram
已经构建并表征了一种用于对单细胞进行高速荧光测量的改进仪器。仪器优化完成与中国仓鼠细胞（CHO系）染色的荧光Feulgen程序使用金胺-0或吖啶。仪器分辨率和文物细胞DNA分布的贡献已被确定。该仪器引入了小于2%的总变异系数的情况下，CHO细胞染色明亮的染料，如吖啶黄，其中光子统计效应是最小的。该仪器的设计使得可以以50 000个细胞/分钟的速率在单细胞基础上研究和定量许多细胞成分或性质中的任何一种。
An improved instrument for the making of high-speed fluorescent measurements on single cells has been constructed and characterized. Instrumental optimization was accomplished with Chinese hamser cells (line CHO) stained by the fluorescent Feulgen procedure using either auramine-0 or acriflavine. Contributions of instrumental resolution and artifacts to cellular DNA distributions have been determined. The instrument introduces a total coefficient of variation of less than 2% for the case of CHO cells stained with bright dyes such as acriflavine, where photon-statistical effects are minimal. The design of the instrument is such that any of a number of cellular constituents or properties can be studied and quantitated on a single-cell basis at a rate of 50 000 cells/min.