Differential response related to genotoxicity between eggplant (Solanum melanogena) skin aqueous extract and its main purified anthocyanin (delphinidin) in vivo
Differential response related to genotoxicity between eggplant (Solanum melanogena) skin aqueous extract and its main purified anthocyanin (delphinidin) in vivo
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DOI：
10.1016/j.fct.2006.11.004
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发表时间：
2007-05-01
影响因子：
4.3
通讯作者：
Salvadori, Daisy M. F.
中科院分区：
文献类型：
--
作者：
Azevedo, Luciana;Alves de Lima, Patricia L.;Salvadori, Daisy M. F.
花青素是植物界中最大的一类水溶性色素。多项研究表明，花青素具有抗氧化能力，并对某些癌细胞的生长具有抑制作用。因此，本研究的目的是使用单细胞（彗星）测定和微核测试来评估从黑素茄（一种可能的花青素新来源）及其主要纯化花青素提取物（飞燕草素）中获得的水提取物的抗突变性/抗原毒性和致突变性/基因毒性。用较高剂量的纯化花青素（10 和 20 mg/kg b.w.）进行预处理可导致环磷酰胺诱导的多染红细胞中微核频率显着降低（p < 0.05）。减少幅度从 48% 到 57% 不等，与浓度无关。不明显：花青素或飞燕草素提取物均发现遗传毒性和致突变性。总而言之，这些结果表明，用花青素（飞燕草素）中存在的特定化合物预处理的小鼠，环磷酰胺诱导的突变发生率较低。这一发现强调了天然着色剂预防突变的潜力，以及遗传毒性评估对于改善健康的适用性。此外，这里提出的结果可能是支持在饮食中使用花青素的另一个论据。 (c) 2006 年，爱思唯尔有限公司出版。
Anthocyanins are the largest group of water-soluble pigments in the plant kingdom. A number of studies have demonstrated that anthocyanins present antioxidant capacity and show inhibitory effects on the growth of some cancer cells. Thus, the goal of this study was to evaluate both the antimutagenicity/antigenotoxicity and mutagenicity/genotoxicity of aqueous extract obtained from the Solanum melanogena, a possible novel source of anthocyanin, and its main purified anthocyanin extract (delphinidin), using the single cell (comet) assay and micronucleus test. Pretreatment with higher doses of the purified anthocyanin (10 and 20 mg/kg b.w.) led to a statistically significant reduction (p < 0.05) in the frequency of micronuclei in polychromatic erythrocytes induced by cyclophosphamide. The pattern of reduction ranged from 48% to 57% independent of concentration. No apparent: genotoxicity and mutagenicity was found for either the anthocyanin or delphinidin extracts. Taken together, these results suggest that mice pre-treated with specific compounds present in anthocyanins (delphinidin) displayed a lower incidence of mutations induced by cyclophosphamide. This finding emphasizes the potential of natural colorants to prevent mutations and also the applicability of genotoxic evaluation for improving health. Furthermore, the results presented here could be an additional argument to support the use of anthocyanins in the diet. (c) 2006 Published by Elsevier Ltd.