Functional tooth restoration utilising split germs through re-regionalisation of the tooth-forming field.
Functional tooth restoration utilising split germs through re-regionalisation of the tooth-forming field.
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DOI：
10.1038/srep18393
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发表时间：
2015-12-17
影响因子：
4.6
通讯作者：
Tsuji T
中科院分区：
文献类型：
--
作者：
Yamamoto N;Oshima M;Tanaka C;Ogawa M;Nakajima K;Ishida K;Moriyama K;Tsuji T
牙齿是一种外胚层器官，在上皮间质相互作用的调节下由牙胚产生。牙齿形态发生发生在牙齿形成领域，是特定基因表达模式的反应扩散波的结果。在这里，我们开发了一种新颖的机械结扎方法，用于分裂牙胚，以人为地调节控制牙齿形态的分子。通过牙齿形成场的重新区域化，分裂的牙胚成功发育成多个正确的牙齿，该区域由响应机械力的反应扩散波调节。此外，裂开的牙齿长出口腔并恢复牙齿的生理功能，包括咀嚼、牙周膜功能和对有害刺激的反应。因此，本研究提出了一种基于分裂牙胚和通过人工机械力重新区域化牙齿形成场的新型牙齿再生技术。
The tooth is an ectodermal organ that arises from a tooth germ under the regulation of reciprocal epithelial-mesenchymal interactions. Tooth morphogenesis occurs in the tooth-forming field as a result of reaction-diffusion waves of specific gene expression patterns. Here, we developed a novel mechanical ligation method for splitting tooth germs to artificially regulate the molecules that control tooth morphology. The split tooth germs successfully developed into multiple correct teeth through the re-regionalisation of the tooth-forming field, which is regulated by reaction-diffusion waves in response to mechanical force. Furthermore, split teeth erupted into the oral cavity and restored physiological tooth function, including mastication, periodontal ligament function and responsiveness to noxious stimuli. Thus, this study presents a novel tooth regenerative technology based on split tooth germs and the re-regionalisation of the tooth-forming field by artificial mechanical force.