Dual-Target Electrochemical Biosensing Based on DNA Structural Switching on Gold Nanoparticle-Decorated MoS2 Nanosheets
Dual-Target Electrochemical Biosensing Based on DNA Structural Switching on Gold Nanoparticle-Decorated MoS2 Nanosheets
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基于金纳米颗粒修饰的 MoS2 纳米片 DNA 结构转换的双靶点电化学生物传感
DOI：
10.1021/acsami.5b12833
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发表时间：
2016-03-23
影响因子：
9.5
通讯作者：
Wang, Lianhui
中科院分区：
文献类型：
--
作者：
Su, Shao;Sun, Haofan;Wang, Lianhui
基于金纳米粒子修饰的MoS2 （AuNPs-MoS2）纳米复合材料，研制了一种同时检测凝血酶和三磷酸腺苷（ATP）的MoS2电化学适体传感器。通过Au-S键将两种不同的适体探针标记为氧化还原标签，同时固定在AuNPs-MoS2膜修饰电极上。随着靶分子（凝血酶和ATP）的加入，适体呈现结构开关，导致亚甲基蓝（MB）远离电极表面或二茂铁（Fc）靠近电极表面。因此，开发了一种双信号检测策略，由于目标诱导的结构切换，在检测系统中具有“信号开启”和“信号关闭”两个元素。该传感器可同时检测低至0.74 nM的ATP和凝血酶，低至0.0012 nM的凝血酶具有高选择性。此外，凝血酶和ATP可以作为输入来激活与逻辑门。
A MoS2-based electrochemical aptasensor has been developed for the simultaneous detection of thrombin and adenosine triphosphate (ATP) based on gold nano particles-decorated MoS2 (AuNPs-MoS2) nanocomposites. Two different aptamer probes labeled with redox tags were simultaneously immobilized on an AuNPs-MoS2 film modified electrode via Au-S bonds. The aptamers presented structural switches with the addition of target molecules (thrombin and ATP), resulting in methylene blue (MB) far from or ferrocene (Fc) close to the electrode surface. Therefore, a dual signaling detection strategy was developed, which featured both "signal-on" and "signal-off' elements in the detection system because of the target-induced structure switching. This proposed aptasensor could simultaneously determine ATP and thrombin as low as 0.74 nM ATP and 0.0012 nM thrombin with high selectivity, respectively. In addition, thrombin and ATP could act as inputs to activate an AND logic gate.