Macrophage phagocytosis of wound neutrophils
Macrophage phagocytosis of wound neutrophils
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DOI：
10.1002/jlb.65.1.35
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发表时间：
1999-01-01
影响因子：
5.5
通讯作者：
Albina, JE
中科院分区：
文献类型：
--
作者：
Meszaros, AJ;Reichner, JS;Albina, JE
急性炎症的消退被认为需要通过受体-配体与巨噬细胞（M phi）的相互作用来识别和吞噬凋亡中性粒细胞（PMN）。在大鼠伤口中，通过定量从损伤后1-3天的伤口获取的新鲜收获的和老化的培养PMN中的细胞凋亡，并通过使用这些伤口PMN作为伤口、免疫激活的腹膜和驻留的腹膜M phi的吞噬靶，来测试该假设。在培养物中老化时，第1天的PMN不发生凋亡，而第2天和第3天的PMN分别有41 +/-1%和29 +/-1%发生凋亡，这与M phi摄入增加相对应。所有三个M phi人口从事不同的受体-配体对的识别和吞噬中性粒细胞。结果表明早期伤口PMN对年龄诱导的细胞凋亡的抵抗，证明伤口-M phi吞噬伤口PMN，并鉴定伤口和其他M phi摄取伤口PMN的不同受体利用。
Resolution of acute inflammation is thought to require the recognition and phagocytosis of apoptotic neutrophils (PMN) through receptor-ligand interactions with macrophages (M phi). This hypothesis was tested in rat wounds by quantifying apoptosis in freshly harvested and aged-in-culture PMN taken from wounds 1-3 days after injury and by using these wound PMN as phagocytic targets for wound, immune-activated peritoneal, and resident peritoneal M phi. Less than 6% of freshly harvested PMN exhibited characteristics of apoptosis, On aging in culture, day 1 PMN did not undergo apoptosis, whereas 41 +/- 1 and 29 +/- 1% of day 2 and 3 PMN, respectively, developed apoptosis, which corresponded to increased ingestion by M phi. All three M phi populations engaged different receptor-ligand pairs for the recognition and phagocytosis of PMN. Results indicate the resistance of early wound PMN to age-induced apoptosis, demonstrate wound-M phi phagocytosis of wound PMN, and identify distinct receptor utilization by wound and other M phi to ingest wound PMN.