Mcm2, but not RPA, is a component of the mammalian early G1-phase prereplication complex.
Mcm2, but not RPA, is a component of the mammalian early G1-phase prereplication complex.
复制标题
DOI：
10.1083/jcb.146.4.709
复制
发表时间：
1999-08-23
期刊：
影响因子：
--
通讯作者：
Gilbert DM
中科院分区：
文献类型：
--
作者：
Dimitrova DS;Todorov IT;Melendy T;Gilbert DM
Previous experiments in Xenopus egg extracts identified what appeared to be two independently assembled prereplication complexes (pre-RCs) for DNA replication: the stepwise assembly of ORC, Cdc6, and Mcm onto chromatin, and the FFA-1–mediated recruitment of RPA into foci on chromatin. We have investigated whether both of these pre-RCs can be detected in Chinese hamster ovary (CHO) cells. Early- and late-replicating chromosomal domains were pulse-labeled with halogenated nucleotides and prelabeled cells were synchronized at various times during the following G1-phase. The recruitment of Mcm2 and RPA to these domains was examined in relation to the formation of a nuclear envelope, specification of the dihydrofolate reductase (DHFR) replication origin and entry into S-phase. Mcm2 was loaded gradually and cumulatively onto both early- and late-replicating chromatin from late telophase throughout G1-phase. During S-phase, detectable Mcm2 was rapidly excluded from PCNA-containing active replication forks. By contrast, detergent-resistant RPA foci were undetectable until the onset of S-phase, when RPA joined only the earliest-firing replicons. During S-phase, RPA was present with PCNA specifically at active replication forks. Together, our data are consistent with a role for Mcm proteins, but not RPA, in the formation of mammalian pre-RCs during early G1-phase.
登录
查看更多内容
DOI：
10.1002/cyto.990120412
发表时间：
1991-01-01
期刊：
CYTOMETRY
影响因子：
--
作者：
BAKKER, PJM;STAP, J;ATEN, J
通讯作者：
ATEN, J
在细胞周期中存在两个细胞周期蛋白/增殖细胞核抗原的种群：与DNA复制位点的关联。
影响因子：
7.8
作者：
Bravo, R;Macdonald-Bravo, H
通讯作者：
Macdonald-Bravo, H
影响因子：
64.8
作者：
Carpenter, PB;Mueller, PR;Dunphy, WG
通讯作者：
Dunphy, WG
影响因子：
11.4
作者：
Coue, M;Kearsey, SE;Mechali, M
通讯作者：
Mechali, M
影响因子：
4.8
作者：
Brosh, RM;Orren, DK;Bohr, VA
通讯作者：
Bohr, VA