VACUUM FREEZE DRYING OF FROZEN SECTIONS FOR DRY-MOUNTING, HIGH-RESOLUTION AUTORADIOGRAPHY.
VACUUM FREEZE DRYING OF FROZEN SECTIONS FOR DRY-MOUNTING, HIGH-RESOLUTION AUTORADIOGRAPHY.
复制标题
冷冻切片的真空冷冻干燥，用于干式、高分辨率放射自显影。
作者：
W. Stumpf;L. J. Roth
不大于1.5×1.5×2 mm的标本在液氮中冷冻，切片，同时仍冷冻，用冷冻切片机。冷冻切片在真空中干燥，然后压在柯达NTB10印版或涂有柯达NTB3乳剂的载玻片上并烘干。用放射性小鼠肝脏进行组织保存试验。用Ha标记的肠黏膜进行放射自显影质量检测。两种组织都保存了良好的细胞学细节，放射自显影在细胞水平上是可以解释的。
Specimens no larger than 1.5 × 1.5 × 2 mm were frozen in liquid nitrogen and sectioned, while still frozen, with a refrigerated microtome. The frozen sections were dried in a vacuum, then pressed onto either Kodak NTB10 plates or onto slides which had been coated with Kodak NTB3 emulsion and dried. Radioactive mouse liver was used to test tissue preservation. Intestinal mucosa with Ha-labeled nuclei was used to test the quality of autoradiography. Good cytological detail was preserved in both tissues, with the autoradiographs interpretable at the cellular level.