Effects of pulsed magnetic stimulation on tumor development and immune functions in mice
Effects of pulsed magnetic stimulation on tumor development and immune functions in mice
复制标题
DOI：
10.1002/bem.20177
复制
发表时间：
2006-01-01
影响因子：
1.9
通讯作者：
Ueno, S
中科院分区：
文献类型：
--
作者：
Yamaguchi, S;Ogiue-Ikeda, M;Ueno, S
我们研究了脉冲磁刺激对小鼠肿瘤发展过程和免疫功能的影响。在实验中使用圆形线圈（内径= 15 mm，外径= 75 mm）。刺激条件为脉冲宽度= 238 μ s，峰值磁场= 0.25 T（线圈中心），频率= 25脉冲/s，1000脉冲/样本/天，小鼠体内磁感应涡电流= 0.79-1.54 A/m（2）。在动物研究中，B 16-BL 6黑色素瘤模型小鼠从注射癌细胞之日起暴露于脉冲磁刺激16天。肿瘤生长研究显示，刺激组中肿瘤重量显著降低（假手术组的54%）。在一项细胞研究中，B I 6-BL 6细胞也暴露于磁场（1000个脉冲/样本，培养皿底部的涡流= 2.36-2.90 A/m（2））;然而，磁感应涡流对细胞活力没有影响。通过测定小鼠脾脏细胞因子的产生来分析脉冲磁刺激后的免疫调节作用。在暴露于上述刺激条件后，小鼠脾脏中肿瘤坏死因子（TNF-α）的产生被显著激活。这些结果显示了应用重复磁刺激所带来的抗肿瘤作用和免疫调节作用的第一个证据，并且还表明了抗肿瘤作用与脉冲磁刺激引起的TNF-α水平增加之间的可能关系。
We investigated the effects of pulsed magnetic stimulation on tumor development processes and immune functions in mice. A circular coil (inner diameter= 15 mm, outer diameter = 75 mm) was used in the experiments. Stimulus conditions were pulse width = 238 mu s, peak magnetic field = 0.25 T (at the center of the coil), frequency = 25 pulses/s, 1000 pulses/sample/day and magnetically induced eddy currents in mice = 0.79-1.54 A/m(2). In an animal study, B 16-BL6 melanoma model mice were exposed to the pulsed magnetic stimulation for 16 days from the day of injection of cancer cells. A tumor growth study revealed a significant tumor weight decrease in the stimulated group (54% of the sham group). In a cellular study, B I 6-BL6 cells were also exposed to the magnetic field (1000 pulses/sample, and eddy currents at the bottom of the dish = 2.36-2.90 A/m(2)); however, the magnetically induced eddy currents had no effect on cell viabilities. Cytokine production in mouse spleens was measured to analyze the immunomodulatory effect after the pulsed magnetic stimulation. tumor necrosis factor (TNF-alpha) production in mouse spleens was significantly activated after the exposure of the stimulus condition described above. These results showed the first evidence of the antitumor effect and immunomodulatory effects brought about by the application of repetitive magnetic stimulation and also suggested the possible relationship between anti-tumor effects and the increase of TNF-alpha levels caused by pulsed magnetic stimulation.