Silk fibroin-based scaffolds for bone regeneration
Silk fibroin-based scaffolds for bone regeneration
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DOI：
10.1002/jbm.b.32839
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发表时间：
2013-02-01
影响因子：
3.4
通讯作者：
Nishiyama, Norihiro
中科院分区：
文献类型：
--
作者：
Kuboyama, Noboru;Kiba, Hideo;Nishiyama, Norihiro
将再生的桑蚕丝素蛋白溶于水或六氟异丙醇（HFIP）中制备多孔支架。观察两种制剂对兔股骨上髁植入后新骨形成和生长的影响。通过骨体积、骨矿物质含量和植入部位的骨矿物质密度判断，水基丝心蛋白（A-F）支架的骨传导性显著高于HFIP丝心蛋白（HFIP-F）支架。显微CT分析表明，新形成的骨的形态在两种类型的丝素蛋白支架中显着不同。植入4周后，在A-F支架植入物的孔内观察到新的骨小梁，而HFIP-F支架仅含有坏死细胞。在后者支架的孔内没有观察到骨小梁，尽管这些支架的孔确实含有巨细胞和肉芽组织。(c)2012 Wiley Periodicals，Inc. J Biomed Mater Res Part B：Appl Biomater，2013.
Porous scaffolds were prepared using regenerated Bombyx mori silk fibroin dissolved in water or hexafluoroisopropanol (HFIP). The effects of these two preparations on the formation and growth of new bone on implantation into the rabbit femoral epicondyle was examined. The aqueous-based fibroin (A-F) scaffold exhibited significantly greater osteoconductivity as judged by bone volume, bone mineral content, and bone mineral density at the implant site than the HFIP-based fibroin (HFIP-F) scaffold. Micro-CT analyses showed that the morphology of the newly formed bone differed significantly in the two types of silk fibroin scaffold. After 4 weeks of implantation, new trabecular bone was seen inside the pores of the A-F scaffold implant while the HFIP-F scaffold only contained necrotic cells. No trabecular bone was seen within the pores of the latter scaffolds, although the pores of these did contain giant cells and granulation tissue. (c) 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2013.