Plasma half-life and tissue distribution of leukocyte cell-derived chemotaxin 2 in mice
Plasma half-life and tissue distribution of leukocyte cell-derived chemotaxin 2 in mice
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DOI：
10.1038/s41598-020-70192-x
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发表时间：
2020-08-06
影响因子：
4.6
通讯作者：
Takamura, Toshinari
中科院分区：
文献类型：
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作者：
Kikuchi, Akihiro;Takayama, Hiroaki;Takamura, Toshinari
白细胞来源的趋化素2 （LECT2）是一种引起骨骼肌胰岛素抵抗的肝因子。LECT2的循环水平是一种可能的生物标志物，可以预测体重循环，因为它们反映肝脏脂肪，在体重减轻或增加之前开始。在此，为了阐明血清LECT2水平快速变化的动力学，我们通过将i -125标记的LECT2注射到ICR小鼠体内并进行放射性示踪，研究了LECT2的体内动力学，包括其血浆半衰期和组织分布。注射的LECT2在10分钟内从血流中消除（大约半衰期，5分钟）。在肾脏中，放射性在注射后10分钟内积累，随后下降。相反，注射30 min后，尿液中的放射性增加，说明LECT2主要由肾脏排泄到尿液中。最后，LECT2在骨骼肌和肝脏中积累，分别持续到注射后30分钟和2分钟。脂肪组织中未观察到LECT2积累。这些发现与LECT2对骨骼肌的作用一致。目前的研究表明，LECT2是一种快速转换的蛋白，这使得LECT2的循环水平成为预测体重变化的有用的快速反应生物标志物。
Leukocyte cell-derived chemotaxin 2 (LECT2) is a hepatokine that causes skeletal muscle insulin resistance. The circulating levels of LECT2 are a possible biomarker that can predict weight cycling because they reflect liver fat and precede the onset of weight loss or gain. Herein, to clarify the dynamics of this rapid change in serum LECT2 levels, we investigated the in vivo kinetics of LECT2, including its plasma half-life and tissue distribution, by injecting I-125-labelled LECT2 into ICR mice and radioactivity tracing. The injected LECT2 was eliminated from the bloodstream within 10 min (approximate half-life, 5 min). In the kidneys, the radioactivity accumulated within 10 min after injection and declined thereafter. Conversely, the radioactivity in urine increased after 30 min of injection, indicating that LECT2 is mainly excreted by the kidneys into the urine. Finally, LECT2 accumulated in the skeletal muscle and liver until 30 min and 2 min after injection, respectively. LECT2 accumulation was not observed in the adipose tissue. These findings are in agreement with LECT2 action on the skeletal muscle. The present study indicates that LECT2 is a rapid-turnover protein, which renders the circulating level of LECT2 a useful rapid-response biomarker to predict body weight alterations.