Development of Molecular Markers Linked to the Allele Associated with the Non-astringent Trait of the Chinese Persimmon (Diospyros kaki Thunb.)
Development of Molecular Markers Linked to the Allele Associated with the Non-astringent Trait of the Chinese Persimmon (Diospyros kaki Thunb.)
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DOI：
10.2503/jjshs1.80.150
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发表时间：
2011-04-01
影响因子：
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通讯作者：
Yonemori, Keizo
中科院分区：
文献类型：
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作者：
Ikegami, Ayako;Eguchi, Sai;Yonemori, Keizo
根据果实失涩的性质，将多年生栽培品种分为4种类型，即授粉常涩型（PCNA）、授粉变异非涩型（PVNA）、授粉变异涩型（PVA）和授粉常涩型（PCA）。在这四种类型中，PCNA是最重要的柿子品种，因为它在树上稳定地失去了自然收敛性。日本PCNA品种的自然失涩性状为隐性，而中国PCNA品种的自然失涩性状为显性，中国PCNA品种的自然失涩性状与日本品种的自然失涩性状不同，中国PCNA品种的自然失涩性状与日本品种的自然失涩性状不同。为了开发一个可用于选择CPCNA型品种的分子标记，以“罗田甜食”为母本，对其F1代进行扩增片段长度多态性（AFLP）和群体分离分析。共检测了384对引物组合，获得了3个与CPCNA显性等位基因连锁的AFLP标记，即EACT-MCCC-222（RO 1）、EGGC-MCTC-309（RO 2）和EGCC-MCGA-105（RO 3）。在这些标记中，EGGC/MCTC-309（RO 2）被转换为序列特征扩增区（SCAR）标记。对264个F1代后代的PCR检测结果表明，该SCAR标记的相关率为94%。RO 2标记与CPCNA显性等位基因紧密连锁，在中国、韩国和日本供试的PCNA品种中，仅在“罗田田氏”和“天宝盖”两个品种中检测到多态性。这些结果表明，RO 2标记有助于分子标记辅助选择具有CPCNA性状的PCNA新品种的育种计划。
Persimmon cultivars are classified into four types depending on the nature of astringency loss of the fruit, namely pollination constant non-astringent (PCNA), pollination variant non-astringent (PVNA), pollination variant astringent (PVA), and pollination constant astringent (PCA). Among these four types, PCNA is the most important cultivar for persimmon breeding due to the stable loss of natural astringency on the tree. The trait of natural astringency loss is recessive in Japanese PCNA cultivars, while that in the Chinese PCNA cultivar, 'Luo tian tian shi', is dominant and the latter locus, termed CPCNA, is different from that in Japanese cultivars. In order to develop a molecular marker for the selection of the CPCNA-type cultivar, we performed amplified fragment length polymorphism (AFLP) in combination with bulked segregant analysis for F-1 offspring derived from 'Luo tian tian shi', which was used as the maternal parent. A total of 384 primer combinations were tested, and three AFLP markers, namely EACT-MCCC-222 (RO1), EGGC-MCTC-309 (RO2), and EGCC-MCGA-105 (RO3), linked to the CPCNA dominant allele were obtained. Among these markers, EGGC/MCTC-309 (RO2) was converted into a sequence-characterized amplified region (SCAR) marker. PCR analysis using F-1 offspring (n = 264) revealed that the relevance ratio of the SCAR marker was 94%. The polymorphism of the RO2 marker, which was strongly linked to the CPCNA dominant allele, was detected in only two Chinese PCNA cultivars, namely 'Luo tian tian shi' and 'Tian bao gai', among the Chinese, Korean, and Japanese cultivars tested. These results indicate that the RO2 marker contributes to marker-assisted selection for breeding programs of new PCNA cultivars having the CPCNA trait.