Systematic screening for palmitoyl transferase activity of the DHHC protein family in mammalian cells
Systematic screening for palmitoyl transferase activity of the DHHC protein family in mammalian cells
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DOI：
10.1016/j.ymeth.2006.05.015
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发表时间：
2006-10-01
期刊：
影响因子：
4.8
通讯作者：
Fukata, Masaki
中科院分区：
文献类型：
--
作者：
Fukata, Yuko;Iwanaga, Tsuyoshi;Fukata, Masaki
翻译后修饰，包括磷酸化、泛素化和脂质修饰，为蛋白质提供了超越基因组信息的额外功能和调节。棕榈酰化是一种与棕榈酸可逆的脂质修饰，在蛋白质转运和功能中起着关键作用。然而，调节棕榈酰转移酶(PAT)的酶一直难以捉摸。最近在酵母中的遗传分析表明，富含半胱氨酸的DHHC结构域的成员(DHHC蛋白)介导棕榈酰化。在哺乳动物基因组中，预测有23种DHHC蛋白，这增加了PAT酶大家族的可能性。在这里，我们描述了一种系统的方法来检查DHHC家族中的哪一个成员负责底物的棕榈酰化。(C)2006 Elsevier Inc.保留所有权利。
Posttranslational modifications, including phosphorylation, ubiquitination and lipid modifications, provide proteins with additional functions and regulation beyond genomic information. Palmitoylation is a reversible lipid modification with palmitic acid that plays critical roles in protein trafficking and function. However, the enzymes that mediate palmitoyl acyl transferase (PAT) have been elusive. Recent genetic analysis in yeast revealed that members of cysteine-rich DHHC domain containing proteins (DHHC proteins) mediate palmitoylation. In mammalian genomes, 23 DHHC proteins are predicted raising the possibility of a large family of PAT enzymes. Here, we describe a systematic method to examine which of the DHHC family members is responsible for palmitoylation of a substrate. (c) 2006 Elsevier Inc. All rights reserved.