A Wide-Field Imaging Approach for Simultaneous Super-Resolution Surface-Enhanced Raman Scattering Bioimaging and Spectroscopy.
A Wide-Field Imaging Approach for Simultaneous Super-Resolution Surface-Enhanced Raman Scattering Bioimaging and Spectroscopy.
复制标题
同时进行超分辨表面增强拉曼散射生物成像和光谱学的宽视场成像方法。
DOI：
10.1021/acsmeasuresciau.2c00013
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发表时间：
2022-08-17
期刊：
影响因子：
--
通讯作者：
Schultz, Zachary D.
中科院分区：
文献类型：
--
作者：
Shoup, Deben N.;Scarpitti, Brian T.;Schultz, Zachary D.
高空间分辨率成像和生物体中的化学特异性检测在从医学到催化的广泛领域中是重要的。在这项工作中，我们描述了一种宽场表面增强拉曼散射（Sers）成像方法，能够同时捕获图像和Sers光谱的纳米粒子Sers标签在癌细胞。在图像到达阵列检测器之前，通过透射衍射光栅传递图像，我们在相机传感器上同时记录图像和波长色散信号。实验的优化提供了一种比通常用于宽场Sers成像的液晶可调谐滤波器具有更好的光谱分辨率和更快速采集的方法。Sers固有的强度波动使得定位算法能够应用于空间域和谱域两者，从而提供与在Sers标签的光谱中识别的改进的峰位置相关的超分辨率Sers图像。检测到的拉曼信号被证明是敏感的焦平面，提供三维（3D）切片检测到的纳米粒子的能力。我们的工作表明，光谱分辨超分辨率Sers成像，有可能被应用到复杂的物理和生物成像应用。
High spatial resolution imaging and chemical-specific detection in living organisms is important in a wide range of fields from medicine to catalysis. In this work, we characterize a wide-field surface-enhanced Raman scattering (SERS) imaging approach capable of simultaneously capturing images and SERS spectra from nanoparticle SERS tags in cancer cells. By passing the image through a transmission diffraction grating before it reaches an array detector, we record the image and wavelength dispersed signal simultaneously on the camera sensor. Optimization of the experiment provides an approach with better spectral resolution and more rapid acquisition than liquid crystal tunable filters commonly used for wide-field SERS imaging. Intensity fluctuations inherent to SERS enabled localization algorithms to be applied to both the spatial and spectral domain, providing super-resolution SERS images that are correlated with improved peak positions identified in the spectrum of the SERS tag. The detected Raman signal is shown to be sensitive to the focal plane, providing three-dimensional (3D) sectioning abilities for the detected nanoparticles. Our work demonstrates spectrally resolved super-resolution SERS imaging that has the potential to be applied to complex physical and biological imaging applications.
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