Autoradiography system with phosphor powder (ZnS:Ag) for imaging radioisotope dynamics in a living plant
Autoradiography system with phosphor powder (ZnS:Ag) for imaging radioisotope dynamics in a living plant
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DOI：
10.35848/1347-4065/ac2b50
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发表时间：
2021-11-01
影响因子：
1.5
通讯作者：
Kawachi, Naoki
中科院分区：
文献类型：
--
作者：
Kurita, Keisuke;Sakai, Takuro;Kawachi, Naoki
放射性同位素示踪成像是研究植物生理现象的有效手段。在这项研究中，我们开发了一种含磷光粉(ZnS：Ag)的放射自显影系统，即“实时放射自显影术”，用于成像活植物中的放射性同位素动力学。该系统直观地显示了光环境下完整植物体内元素的连续迁移和积累过程。对Cs-137的点源进行了成像测试，观察到的放射性为10-100kBq；这表明成像强度与Cs-137的放射性之间具有令人满意的系统线性。此外，还对一株完整的大豆植株进行了为期四天的Cs-137动态成像。系列图像显示，Cs-137在植物的节部、叶脉和生长点积累。该系统可用于研究植物生理现象，并可用于放射性核素的定量测量。
Radioisotope tracer imaging is useful for studying plant physiological phenomena. In this study, we developed an autoradiography system with phosphor powder (ZnS:Ag), "Live-autoradiography", for imaging radioisotope dynamics in a living plant. This system visualizes the element migration and accumulation in intact plants continuously under a light environment. An imaging test was performed on point sources of Cs-137, with radioactivity of 10-100 kBq of being observed; this indicates satisfactory system linearity between the image intensity and the radioactivity of Cs-137. Moreover, dynamics imaging of Cs-137 was performed on an intact soybean plant for four days. The serial images indicated Cs-137 accumulation in the node, vein, and growing point of the plant. The developed system can be used for studying plant physiological phenomena and can be employed for the quantitative measurement of radionuclides.