Wheat Rust Surveillance: Field Disease Scoring and Sample Collection for Phenotyping and Molecular Genotyping

Wheat Rust Surveillance: Field Disease Scoring and Sample Collection for Phenotyping and Molecular Genotyping
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DOI:
10.1007/978-1-4939-7249-4_1
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发表时间:
2017-01-01
期刊:
WHEAT RUST DISEASES: METHODS AND PROTOCOLS
影响因子:
--
通讯作者:
Hodson, David
Hodson, David
中科院分区:
其他
文献类型:
--
作者:
Ali, Sajid;Hodson, David

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长距离迁移能力、入侵谱系的出现以及对各种气候条件的适应能力的可变性使小麦锈病成为全球小麦生产的最重要威胁。需要有效和协调的努力来监测不同地理层面的病原体种群,以便能够在地方、区域、大陆乃至全球范围内跟踪锈病病原体种群。在这里,我们描述了锈病监测的标准程序,以便能够在不同研究小组之间进行比较,以形成最终的汇编。所描述的程序将能够跟踪疾病的严重程度、宿主抗性的现场水平表达以及收集样本以进行进一步的毒力表型和分子基因分型。
Long-distance migration capacity, emergence of invasive lineages, and variability in adaptation to a wide range of climatic conditions make wheat rusts the most important threat to wheat production worldwide. Efficient and coordinated efforts are required for surveillance of the pathogen population at different geographical levels to enable tracking of rust pathogen populations at local, regional, continental, and ultimately worldwide scale. Here we describe a standard procedure for rust surveillance to enable comparison across various research groups for a final compilation. The procedure described would enable tracking of disease severity, field level expression of host resistance, and collection of samples for further virulence phenotyping and molecular genotyping.