VdTps2 Modulates Plant Colonization and Symptom Development in Verticillium dahliae
VdTps2 Modulates Plant Colonization and Symptom Development in Verticillium dahliae
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DOI：
10.1094/mpmi-03-23-0024-r
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发表时间：
2023-09-01
影响因子：
3.5
通讯作者：
Wang，Yonglin
中科院分区：
文献类型：
--
作者：
Xiao，Luyao;Tang，Chen;Wang，Yonglin
海藻糖生物合成途径是抗真菌药物开发的潜在靶点。海藻糖磷酸合酶（TPS）和磷酸酶是真菌海藻糖生物合成中广泛保守的组分。然而，海藻糖的生物合成在维管植物致病菌大丽花黄萎病菌中的作用尚不清楚。本文研究了大丽花中VdTps1、VdTps2和VdTps3三种TPS复合物的功能。与evdtps2不同，与野生型菌株相比，vdtps1或vdtps3的缺失没有改变任何表型。与此相反，ΔVdTps2strain由于菌丝尖端的异常膨胀，其径向生长受到严重抑制。此外，vdtps2的缺失增加了微核的形成、黑色素的生物合成以及对细胞壁扰动和高温胁迫的抵抗力。毒力测定和真菌生物量定量显示，vdtps2的缺失延迟了疾病症状的发展，接种后植物茎组织中ΔVdTps2strain的毒力降低和生物量减少。此外，在h2o2存在下，在ΔVdTps2strain中观察到穿透性peg形成的增加，这表明VdTps2抑制了初始定殖。我们的研究结果还揭示了vdtps2作为自噬调节因子的作用。总之，这些结果表明vdtps2有助于植物定植和疾病发展。版权所有©2023作者。这是一篇在CC BY-NC-ND 4.0国际许可下发布的开放获取文章。
The trehalose biosynthesis pathway is a potential target for antifungal drugs development. Trehalose phosphate synthase (TPS) and phosphatase are widely conserved components of trehalose biosynthesis in fungi. However, the role of trehalose biosynthesis in the vascular plant-pathogenic fungusVerticillium dahliaeremains unclear. Here, we investigated the functions of the TPS complex, including VdTps1, VdTps2, and VdTps3 inV. dahliae. UnlikeVdTps2, deletion ofVdTps1orVdTps3did not alter any phenotypes compared with the wild-type strain. In contrast, the ΔVdTps2strain showed severely depressed radial growth due to the abnormal swelling of the hyphal tips. Further, deletion ofVdTps2increased microsclerotia formation, melanin biosynthesis, and resistance to cell-wall perturbation and high-temperature stress. Virulence assays and quantification of fungal biomass revealed that deletion ofVdTps2delayed disease symptom development, as evident by the reduced virulence and decreased biomass of the ΔVdTps2strain in plant stem tissue following inoculation. Additionally, increases in penetration peg formation observed in the ΔVdTps2strain in the presence of H2O2suggested that VdTps2 suppresses initial colonization. Our results also revealed the role ofVdTps2as a regulator of autophagy. Together, these results indicate thatVdTps2contributes to plant colonization and disease development.Copyright © 2023 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.