A reconstituted in vitro clot model for evaluating laser thrombolysis
A reconstituted in vitro clot model for evaluating laser thrombolysis
复制标题
DOI：
10.1023/a:1020431007864
复制
发表时间：
2002-06-01
影响因子：
4
通讯作者：
Gregory, K
中科院分区：
文献类型：
--
作者：
Janis, AD;Buckley, LA;Gregory, K
背景/目的：激光溶栓是一种利用激光能量选择性去除闭塞血管血栓的方法。建立了具有可重复光学吸收特性的重建血块模型，以评估各种激光参数对血栓去除率的影响。研究设计/材料和方法：用已知的纤维蛋白原浓度和血细胞比容制成重建凝块。从10头猪身上采集离体血块。制备了4个红色凝胶模型。使用577 nm， 1 musec脉冲染料激光器测定质量去除率和烧蚀效率。在能量为25 mJ、重复频率为4 Hz的条件下，比较三种凝块模型的消融效率。此外，在平均功率保持在100 mW不变的情况下，改变脉冲能量和重复频率，对重建的凝块模型进行烧蚀。结果：离体凝块的平均消融效率为0.4 +/- 0.1 ~ 3.4 +/- 0.7马克杯/mJ/脉冲，组间差异有统计学意义（方差分析p < 0.05）。在此能量和重复率下，不同纤维蛋白原含量的重构凝块的消融效率为1.5 +/- 0.2至1.6 +/- 0.3 μg/mJ/脉冲。明胶消融效率与蛋白质含量成反比，范围为0.5 +/- 0.3 ~ 2.0 +/- 0.7 μg/mJ/脉冲。在13 mJ （8 Hz）和33 mJ （3 Hz）的设置范围内，重建凝块的去除率（以μg/s为单位）在临床上相似。结论：构建的模型血块具有可重复性和生物学相关性。离体凝块在个体之间缺乏可重复性，明胶幻影缺乏临床相关性。在恒定的平均功率下，不同的激光参数没有影响肿块去除率到临床显著程度。
Background/Objective: Laser thrombolysis is the selective removal of thrombus from occluded blood vessels using laser energy. A reconstituted clot model with reproducible optical absorption properties was developed to evaluate the effect of various laser parameters on thrombus removal rate.Study Design/Materials and Methods: Reconstituted clots were made with known fibrinogen concentrations and hematocrits. Ex vivo clots were collected from ten swine. Four red gelatin phantoms were prepared. Mass removal rates and ablation efficiencies were determined using a 577 nm, 1 musec pulsed dye laser. The ablation efficiencies of the three clot models were compared at an energy of 25 mJ and a repetition rate of 4 Hz. In addition, the reconstituted clot model was ablated as pulse energy and repetition rate were varied with average power held constant at 100 mW.Results: The mean ablation efficiency for ex vivo clots ranged from 0.4 +/- 0.1 to 3.4 +/- 0.7 mug/mJ/pulse, with significant differences between groups (ANOVA p < 0 05). Reconstituted clots of varied fibrinogen content had ablation efficiencies of 1.5 +/- 0.2 to 1.6 +/- 0.3 μg/mJ/pulse at this energy and repetition rate. Gelatin ablation efficiency was inversely proportional to protein content and ranged from 0.5 +/- 0.3 to 2.0 +/- 0.7 μg/mJ/pulse. Reconstituted clot mass removal rates (in μg/s) were clinically similar for settings ranging from 13 mJ at 8 Hz to 33 mJ at 3 Hz.Conclusions: The reconstituted model clot is a reproducible and biologically relevant thrombolysis target. Ex vivo clot lacks reproducibility between individuals and gelatin phantoms lack clinical relevance. At a constant average power, varying laser parameters did not affect mass removal rates to a clinically significant degree.