Static inflation attenuates ischemia/reperfusion injury in an isolated rat lung in situ
Static inflation attenuates ischemia/reperfusion injury in an isolated rat lung in situ
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DOI：
10.1378/chest.126.2.552
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发表时间：
2004-08-01
期刊：
影响因子：
9.6
通讯作者：
Chen, HI
中科院分区：
文献类型：
--
作者：
Kao, SJ;Wang, D;Chen, HI
研究目的：缺血(1)/再灌注(R)肺损伤是肺移植的重要临床问题。在本研究中，我们观察了肺静态膨胀、不同的灌流液以及含氮或含氧的呼吸机气体对肺I/R损伤的影响。设计和地点：选用雄性SD大鼠96只。方法：通过测定或计算肺毛细血管滤过系数(KFC)、肺增重(LWG)、肺重量/体重(LW/BW)比值、保管液蛋白浓度(PCBAL)来评价肺损伤程度。结果：I/R引起肺损伤，表现为KFC、LWG、LW/BW和PCBAL的增加。组织病理学检查可见透明膜形成，炎性细胞浸润。这些值被静态肺充气显著减弱。全血灌注组的IR肺损伤明显轻于等渗液灌注组。结论：肺充气对I/R损伤有保护作用，与治疗所用的通风空气类型无关。供移植用的肺的保存最好保持在静态充气状态，并用全血而不是等渗生理溶液进行灌流。
Study objectives: Ischemia (1)/reperfusion (R) lung injury is an important clinical issue in lung transplantation. In the present study, we observed the effects of lung static inflation, different perfusates, and ventilatory gas with nitrogen or oxygen on the I/R-induced pulmonary damage.Design and setting: A total of 96 male Sprague-Dawley rats were used. The lung was isolated in Situ.Methods: In an isolated lung, the capillary filtration coefficient (Kfc), lung weight gain (LWG), lung weight (LW)/body weight (BW) ratio, and protein concentration in BAIL fluid (PCBAL) were measured or calculated to evaluate the degree of lung injury. Histologic examinations with hematoxylin-eosin staining were performed.Results: I/R caused lung injury, as reflected by increases in Kfc, LWG, LW/BW, and PCBAL. The histopathologic picture revealed the presence of hyaline membrane formation and the infiltration of inflammatory cells. These values were significantly attenuated by static lung inflation. The IR lung damage appeared to be less in the lung perfused with whole blood than in the lung perfused with an isotonic solution. Therapy with ventilatory air (ie, nitrogen or oxygen) did not alter the I/R lung damage.Conclusions: The data suggest that lung inflation is protective to I/R injury, irrespective of the type of ventilatory air used for treatment. The preservation of the lung for transplantation is better kept at a static inflation state and perfused with whole blood instead of an isotonic physiologic solution.