CYTOKINE EXPRESSION IN HUMAN PRIMARY AND METASTATIC MELANOMA-CELLS - ANALYSIS IN FRESH BIOPTIC SPECIMENS
CYTOKINE EXPRESSION IN HUMAN PRIMARY AND METASTATIC MELANOMA-CELLS - ANALYSIS IN FRESH BIOPTIC SPECIMENS
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DOI：
10.1097/00008390-199502000-00005
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发表时间：
1995-02-01
影响因子：
2.2
通讯作者：
BIANCHISCARRA, G
中科院分区：
文献类型：
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作者：
CIOTTI, P;RAINERO, ML;BIANCHISCARRA, G
近年来，多项研究证实黑色素瘤细胞系可产生多种细胞因子/生长因子及其受体。由于细胞系可以获得改变的属性，如生长要求的变化，我们研究了来自20个新鲜手术标本的黑色素瘤细胞的构成细胞因子基因的表达：7个原发黑色素瘤和13个转移瘤(12个淋巴结转移)和1个皮下转移瘤)。采用不连续梯度法分离肿瘤细胞后，用逆转录聚合酶链式反应检测肿瘤细胞的mRNA表达。大多数黑色素瘤细胞表达生长因子：碱性成纤维细胞生长因子、白介素1α、白介素1β、白介素6和白介素8，在20例黑色素瘤细胞中有5例表达粒细胞巨噬细胞集落刺激因子(2/5也呈GM-CSF受体阳性)。我们的结果并不表明细胞因子的表达与获得活检标本时的临床分期有直接关系。然而，它们允许我们提出一种可能的转移性肿瘤细胞表型，在这种表型中，自体GM-CSF表达可以调节对肿瘤细胞本身的免疫反应，或者可以增强转移性定植特性。
In recent years, several studies have documented that melanoma cell lines produce various cytokine/growth factors and their receptors. Since cell lines can acquire altered properties, such as changes in growth requirements, we studied constitutive cytokine gene expression in melanoma cells from 20 fresh surgical specimens: seven primary melanomas and 13 metastases (12 lymph-node metastases) and one subcutaneous metastasis). After tumor cell isolation by discontinuous gradient, we tested for mRNA expression by means of reverse-transcriptase polymerase chain reaction. Most melanoma cells tested expressed growth factors: basic fibroblast growth factor (bFGF), interleukin (IL) 1 alpha, IL-1beta, IL-6 and IL-8 and, in five cases out of 20, expressed granulocyte-macrophage colony-stimulating factor (GM-CSF) (two out of five were also positive for GM-CSF receptor). Our results do not point to a direct correlation between cytokine expression and clinical stage at the time when the bioptic specimen was obtained. However, they allow us to suggest a possible metastatic tumour cell phenotype, in which autogenous GM-CSF expression could modulate immune response against the tumour cell itself or could potentiate metastatic colonization properties.