Versatile Microfluidics for Biofabrication Platforms Enabled by an Agile and Inexpensive Fabrication Pipeline.
Versatile Microfluidics for Biofabrication Platforms Enabled by an Agile and Inexpensive Fabrication Pipeline.
复制标题
用于生物制造平台的多功能微流体通过灵活且廉价的制造管道实现。
DOI：
10.1002/adhm.202300636
复制
发表时间：
2023
影响因子：
10
通讯作者：
Moetazedian A
中科院分区：
文献类型：
--
作者：
Moetazedian A
微流体技术已经改变了再生医学的诊断和筛选。最近，它们在生物制造业中显示出很大的前景。然而，它们的采用受到昂贵且耗时的光刻工艺的抑制，从而限制了进展。在这里，具有复杂核壳几何形状的多材料纤维，其尺寸与人体动脉和小动脉的尺寸相匹配，采用灵活且廉价的制造流水线生产的多功能微流体设备制造。该管道包括材料挤出增材制造，采用创新的连续变化挤出（CONVEX）方法生产具有复杂无缝几何形状的微流体，包括通道宽度范围为100-400 µm的新型可变宽度锯齿形（V-zigzag）混合器和流体动力学流动聚焦组件。微流体系统通过在特定区域减速流体以增加跨界面的扩散来促进流体的快速混合。即使在高流速（100 - 1000 µL min-1）下也能更好地混合，同时避免湍流，即使使用100 µm喷嘴也能获得高细胞相容性（>86%）。所展示的3D打印微流体系统是通用的，简单而高效的，为显着推进再生医学中的微流体平台提供了巨大的潜力。
Microfluidics have transformed diagnosis and screening in regenerative medicine. Recently, they are showing much promise in biofabrication. However, their adoption is inhibited by costly and drawn‐out lithographic processes thus limiting progress. Here, multi‐material fibers with complex core‐shell geometries with sizes matching those of human arteries and arterioles are fabricated employing versatile microfluidic devices produced using an agile and inexpensive manufacturing pipeline. The pipeline consists of material extrusion additive manufacturing with an innovative continuously varied extrusion (CONVEX) approach to produce microfluidics with complex seamless geometries including, novel variable‐width zigzag (V‐zigzag) mixers with channel widths ranging from 100–400 µm and hydrodynamic flow‐focusing components. The microfluidic systems facilitated rapid mixing of fluids by decelerating the fluids at specific zones to allow for increased diffusion across the interfaces. Better mixing even at high flow rates (100−1000 µL min−1) whilst avoiding turbulence led to high cell cytocompatibility (>86%) even when 100 µm nozzles are used. The presented 3D‐printed microfluidic system is versatile, simple and efficient, offering a great potential to significantly advance the microfluidic platform in regenerative medicine.