Effect of amalgam fillings on the mercury concentration in human amniotic fluid
Effect of amalgam fillings on the mercury concentration in human amniotic fluid
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DOI：
10.1007/s00404-003-0578-6
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发表时间：
2005-02-05
影响因子：
2.6
通讯作者：
Dessole, Salvatore
中科院分区：
文献类型：
--
作者：
Luglie, Pier Franca;Campus, Guglielmo;Dessole, Salvatore
背景：甲基汞（MeHg）和金属汞是众所周知的神经毒剂。牙科汞合金是普通人群中元素汞蒸气暴露的重要原因。孕妇羊水中汞的浓度及其对胎儿的潜在毒性作用尚不清楚。目的：主要是评估人类AF中可检测汞（Hg）浓度的存在与孕妇汞合金填充物的数量和表面积之间的关系;其次是分析其产科史和围产期并发症。方法：对72例孕妇进行前瞻性研究。一名牙医记录了牙齿状况、汞合金填充物的存在、数量和表面积。采用全自动冷原子吸收仪测定了AF中总汞的含量。根据空白读数确定的AF中汞的检测限为0.08 ng/ml。为了估计解释变量（如汞合金填充物的数量和表面积、鱼类消费、肝脏或神经系统疾病的存在以及吸烟习惯）对汞浓度的依赖性，建立了几个线性回归模型。对总样本和至少有一次汞合金充填的患者（阳性充填组= PF）进行逐步逻辑回归分析。主成分分析（PCA）提供了两个因素，解释了60%以上的变量之间的方差。结果：所有患者AF中的总体平均汞浓度为0.37 +/- 0.49 ng/ml。19名（26.4%）妇女汞浓度< 0.08 ng/ml（汞阴性组）。在53例（73.6%）汞浓度>= 0.08 ng/ml（汞阳性组）患者中，汞平均值为0.49 ± 0.52 ng/ml。汞合金填充物的平均数量在汞阴性组中为2.26 +/- 3.19，在汞阳性组中为5.32 +/- 3.03（ANOVA单因素p=0.04）。汞浓度依赖于汞合金填充物的数量（p=0.03），汞合金填充物的表面积（p=0.04）和鱼的消费量（p=0.04），但没有显着的水平。在逐步logistic过程中，汞合金填充物的数量对模型有贡献（p=0.04），尽管置信区间中包含零值。Hg浓度<0.0 8 ng/ml（n=19）和> 0.0 8 ng/ml（n= 5 3）的孕妇，其产科史和围生期并发症差异无统计学意义（Q2检验）。结论：汞合金充填物的数量和表面积对AF中汞浓度有积极影响，但没有达到显著水平。此外，在房颤中检测到的汞水平较低，在怀孕和新生儿中未观察到不良后果。
Background: Methyl mercury (MeHg) and metallic Hg are well known as neurotoxic agents. Dental amalgam contributes significantly to elemental Hg vapour exposure in the general population. There is little information about Hg concentration in human amniotic fluid (AF) of pregnant women and its potential toxic effect on the fetuses. Objective: Primary to assess the relationship between the presence of detectable mercury (Hg) concentration in human AF, number and surface areas of amalgam fillings of pregnant women; secondary to analyse their obstetric history and perinatal complications. Methods: Seventy-two pregnant women took part in this prospective study. One dentist recorded the dental status, presence, number and surface areas of amalgam fillings. Total Hg concentration in AF was determined in digested samples using automatic cold vapour atomic absorption equipment. The detection limit of Hg in AF, determined from blank readings, was 0.08 ng/ml. To estimate the dependence of the explanatory variables (such as number and surface areas of amalgam fillings, fish consumption, presence of liver or neurological diseases and smoking habits) on mercury concentration several linear regression models were built up. Stepwise logistic regression procedures were running on total sample and on patients with at least one amalgam filling (Positive Filling group = PF). Principal component analysis (PCA) provided two factors, which explained for more the 60% of the variance among the variables. Results: The overall mean Hg concentration in AF among all patients was 0.37 +/- 0.49 ng/ml. Nineteen (26.4%) women had a Hg concentration < 0.08 ng/ml (Hg negative group). In 53 (73.6%) patients, with a concentration >= 0.08 ng/ml (Hg positive group), the mean value of Hg was 0.49 +/- 0.52 ng/ml. The average number of amalgam fillings was 2.26 +/- 3.19 in the Hg negative group and 5.32 +/- 3.03 in the Hg,positive group (ANOVA one-way p=0.04). A dependence of mercury concentration on number of amalgam fillings (p=0.03), surface area of the amalgam fillings (p=0.04) and fish consumption (p=0.04) was observed but not at a significant level. In stepwise logistic procedure the number of amalgam fillings gave a contribution to the model (p=0.04), although null value was included in the confidence intervals. We observed no statistically significant differences Q 2 test) among the patients with a Hg concentration < 0.08 ng/ml (n=19) and those with a concentration >= 0.08 (n=53) with regard to obstetric history and perinatal complications. Conclusions: Number and surface areas of amalgam fillings influenced positively Hg concentration in AF but not at a significant level. Moreover Hg levels detected in AF were low and no adverse outcomes were observed through pregnancies and in the newborns.