Quantitative neuropeptidomics study of the effects of temperature change in the crab Cancer borealis.
Quantitative neuropeptidomics study of the effects of temperature change in the crab Cancer borealis.
复制标题
温度变化对北极蟹影响的定量神经肽组学研究。
DOI：
10.1021/pr500742q
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发表时间：
2014-12-05
影响因子：
4.4
通讯作者：
Li, Lingjun
中科院分区：
文献类型：
--
作者：
Chen, Ruibing;Xiao, Mingming;Buchberger, Amanda;Li, Lingjun
关键词：
温度变化会影响所有生物过程的反应速率，这可能对冷血生物构成巨大挑战，适应温度波动的能力对这些动物的生存至关重要。为了了解神经肽在温度应激反应中的作用，我们采用了基于质谱的方法来研究与急性温度升高相关的神经肽的变化，从Jonah蟹Cancer borealis的三个神经组织。在高温下，观察到来自两个神经肽家族的成员，包括RFamide和RYamide，在其中一个神经内分泌结构（心包器官）中显著减少，而在另一个主要神经内分泌器官（窦腺）中检测到几种orcokinin肽减少。这些结果表明，所观察到的神经肽可能通过激素调节参与温度扰动反应。此外，在热扰动下的动物循环液（血淋巴）中检测到一个一级序列为SFRRMGGKAQ（m/z 1137.7）的温度应激标记肽，并进行了从头测序。
Temperature changes influence the reaction rates of all biological processes, which can pose dramatic challenges to cold-blooded organisms, and the capability to adapt to temperature fluctuations is crucial for the survival of these animals. In order to understand the roles that neuropeptides play in the temperature stress response, we employed a mass spectrometry-based approach to investigate the neuropeptide changes associated with acute temperature elevation in three neural tissues from the Jonah crab Cancer borealis. At high temperature, members from two neuropeptide families, including RFamide and RYamide, were observed to be significantly reduced in one of the neuroendocrine structures, the pericardial organ, while several orcokinin peptides were detected to be decreased in another major neuroendocrine organ, the sinus gland. These results implicate that the observed neuropeptides may be involved with temperature perturbation response via hormonal regulation. Furthermore, a temperature stress marker peptide with the primary sequence of SFRRMGGKAQ (m/z 1137.7) was detected and de novo sequenced in the circulating fluid (hemolymph) from animals under thermal perturbation.
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影响因子：
4.3
作者：
Chen R;Li L
通讯作者：
Li L
DOI：
10.1007/s00359-006-0162-1
发表时间：
2006-12-01
影响因子：
2.1
作者：
Camacho, Joseph;Qadri, Syed Aman;Worden, Mary Kate
通讯作者：
Worden, Mary Kate
DOI：
10.1007/s002440010093
发表时间：
2000-08-01
影响因子：
4
作者：
Lorenzon, S;Francese, M;Ferrero, EA
通讯作者：
Ferrero, EA
DOI：
10.1016/j.bbrc.2011.07.131
发表时间：
2011-09-09
影响因子：
3.1
作者：
Collin, Caitlin;Hauser, Frank;Grimmelikhuijzen, Cornelis J. P.
通讯作者：
Grimmelikhuijzen, Cornelis J. P.
影响因子：
2.5
作者：
Fort, Timothy J.;Brezina, Vladimir;Miller, Mark W.
通讯作者：
Miller, Mark W.