Two-photon fluorescence excitation cross sections of biomolecular probes from 690 to 960 nm
Two-photon fluorescence excitation cross sections of biomolecular probes from 690 to 960 nm
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DOI：
10.1364/ao.37.007352
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发表时间：
1998-11-01
期刊：
影响因子：
1.9
通讯作者：
Webb, WW
中科院分区：
文献类型：
--
作者：
Albota, MA;Xu, C;Webb, WW
我们报告了五种广泛使用的分子荧光团的双光子荧光激发（TPE）作用截面。通过使用超短（类似于100-fs）Ti：蓝宝石脉冲激发在690-960 nm范围内进行测量。通过与荧光素校准标准品比较获得TPE光谱。在700 nm [1 GM = 10（-50）（cm（4）s）/光子]处发现了花菁试剂Cy 3（类似于140 GM）和罗丹明6 G（类似于150 GM）的大截面。几个荧光团显示出有趣的和理想的蓝移相对于单光子吸收波长的两倍。荧光团的荧光强度没有显示出与二次照明功率依赖性的显著偏离（+/-4%），表明真正的双光子过程。这些测量的影响，双光子激光扫描显微镜进行了讨论。(C)1998年美国光学学会OCIS代码：170.2520、190.1900、190.4180。
We report on two-photon fluorescence excitation (TPE) action cross sections for five widely used molecular fluorophores. Measurements were performed by use of ultrashort (similar to 100-fs) Ti:sapphire pulsed excitation over the range 690-960 nm. TPE spectra were obtained by comparison with a fluorescein calibration standard. Large cross sections were found for the cyanine reagent Cy 3 (similar to 140 GM) and for Rhodamine 6G (similar to 150 GM), both at 700 nm [1 GM = 10(-50) (cm(4) s)/photon]. Several fluorophores show interesting and desirable blue shifts with respect to twice the one-photon absorption wavelength. Fluorophore fluorescence intensities showed no significant departure (+/-4%) from quadratic illumination power dependence, indicating genuine two-photon processes. Implications of these measurements for two-photon laser-scanning microscopy are discussed. (C) 1998 Optical Society of America OCIS codes: 170.2520, 190.1900, 190.4180.