Facile synthesis of new periodic mesoporous organosilica and its performance of immobilizing horseradish peroxidase
Facile synthesis of new periodic mesoporous organosilica and its performance of immobilizing horseradish peroxidase
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新型周期性介孔有机二氧化硅的简易合成及其固定化辣根过氧化物酶的性能
DOI：
10.1016/j.micromeso.2012.01.014
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发表时间：
2012-06
影响因子：
5.2
通讯作者：
Zhu, Jian Hua
中科院分区：
文献类型：
--
作者：
Chen, Zhe;Wang, Yi Kai;Wang, Ying;Zhu, Jian Hua
以新型桥联有机硅为前驱体，在酸催化和无机盐辅助的条件下，成功合成了一类含有-OH、-O-和-S-等多种亲水基团的新型周期性介孔有机硅（PMO）。通过XRD、N2吸附、TGA、FT-IR、TEM和NMR等手段对材料进行了表征，结果表明，杂化材料具有较好的介孔结构、较厚的骨架壁、较好的热稳定性和特定的功能基团。此外，首次将辣根过氧化物酶（HRP）固定在这些新型PMO复合材料上，与溶液中游离HRP相比，它们在1，3，5-三甲基苯（TMB）氧化中的酶活性提高了25-59%。同样，HRP固定化的样品可以重复使用许多循环，这是由于当酶附着到充满有机基团和疏水部分的杂交宿主表面时，酶的界面活化。
A new class of periodic mesoporous organosilicas (PMOs), which contains various hydrophilic groups of –OH, –O– and –S–, is successfully synthesized with novel bridged organosilica precursor under the acid-catalyzed and inorganic-salt-assisted conditions. These hybrid materials possess the ordered mesostructure with uniform pore size distributions, thick framework walls, thermal stability and specific functional groups; and they are carefully characterized by XRD, N2adsorption, TGA, FT-IR, TEM and NMR spectroscopies. Moreover, horseradish peroxidase (HRP) is immobilized on these new PMO composites for the first time, and their enzyme activity in the oxidation of 1,3,5-trimethylbenzene (TMB) is elevated in the range of 25–59% compared with that of free HRP in solution. Again, the HRP immobilized samples can be reused for many cycles, resulting from the interfacial activation of the enzyme when it is attached to the surface of hybrid hosts where is filled with organic groups and hydrophobic parts.
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影响因子：
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作者：
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通讯作者：
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影响因子：
8.6
作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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