DIFFERENTIAL REGULATION OF PHENYLALANINE AMMONIA-LYASE GENES DURING ANTHOCYANIN SYNTHESIS AND BY TRANSFER EFFECT IN CARROT CELL SUSPENSION CULTURES
DIFFERENTIAL REGULATION OF PHENYLALANINE AMMONIA-LYASE GENES DURING ANTHOCYANIN SYNTHESIS AND BY TRANSFER EFFECT IN CARROT CELL SUSPENSION CULTURES
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胡萝卜细胞悬浮培养物中花青素合成过程中苯丙氨酸解氨酶基因的差异调控和转移效应
DOI：
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发表时间：
1990
期刊：
影响因子：
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通讯作者：
Yoshiyuki Tanaka
中科院分区：
文献类型：
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作者：
Yoshihiro Qzeki;K. Matsui;M. Sakuta;M. Matsuoka;Y. Ohashi;Y. Kano;N. Yamamoto;Yoshiyuki Tanaka
苯丙氨酸解氨酶（PAL; EC 4.3.1.5）催化类苯丙烷途径中的第一步，并且在分化期间和通过各种刺激诱导。以胡萝卜（Daucus carota L.）CV. Kurodagasun）悬浮培养细胞中，PAL在花青苷合成期间被缓慢诱导，所述花青苷合成发生在缺乏2，4-二氯苯氧基乙酸的培养基中，并且还通过将细胞转移和稀释到新鲜培养基中而被快速和瞬时地诱导。对PAL cDNA序列的分析表明，转移诱导的PAL mRNA转录自不同的胡萝卜PAL基因，而不是花色素苷合成过程中诱导的PAL mRNA。利用PAL cDNA 3 '端非编码区探针进行北方杂交，证实了不同PAL基因在花青苷合成过程中和转移后受到诱导。不同PAL基因的诱导响应于诱导触发物的差异而发生。
Phenylalanine ammonia-lyase (PAL; EC 4.3.1.5) catalyses the first step in the phenylpropanoid pathway and is induced during differentiation and by various stimuli. In carrot (Daucus carota L. cv. Kurodagasun) suspension culture cells, PAL is slowly induced during anthocyanin synthesis which occurs in a medium lacking 2,4-dichlo-rophenoxyacetic acid and is also induced rapidly and transiently by transferring and diluting cells to fresh medium. Analyses of nucleotide sequences derived from PAL cDNAs revealed that the PAL mRNAs induced by transfer were transcribed from different carrot PAL genes than the PAL mRNAs induced during anthocyanin synthesis. Northern blotting using probes derived from 3’non-coding regions for PAL cDNAs confirmed that different PAL genes were induced during anthocyanin synthesis and after transfer. Induction of different PAL genes occurs in response to differences in the induction trigger.
DOI：
10.1073/pnas.80.5.1194
发表时间：
1983-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
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作者：
YOUNG, RA;DAVIS, RW
通讯作者：
DAVIS, RW