Quantification of high-density-lipoprotein cholesterol by precipitation with phosphotungstic acid/MgCl2.
Quantification of high-density-lipoprotein cholesterol by precipitation with phosphotungstic acid/MgCl2.
复制标题
通过磷钨酸/MgCl2 沉淀来定量高密度脂蛋白胆固醇。
作者：
G. Assmann;H. Schriewer;Gerd Schmltz;Edgar
我们评估了使用改良的磷钨酸/MgCl2 沉淀程序沉淀含载脂蛋白 B 的脂蛋白。这些脂蛋白[极低密度和低密度脂蛋白以及脂蛋白(a)]的沉淀是完全的，高密度脂蛋白亚组分（HDL1、HDL2、HDL3）的共沉淀可以忽略不计，即使在高甘油三酯血清中也是如此。与超速离心相比，沉淀法产生的胆固醇值平均低 0.17 mmol/L，但载脂蛋白 A-I 和磷脂酰胆碱的值几乎相同。我们使用“高效”液相色谱法和“高效”薄层色谱法寻找沉淀残留物中由胆固醇形成的δ 3,5-胆甾二烯，但没有发现。
We evaluated the use of a modified phosphotungstic acid/MgCl2 precipitation procedure for the precipitation of apolipoprotein B-containing lipoproteins. Precipitation of these lipoproteins [very-low- and low-density lipoproteins, and lipoprotein (a)] is complete, with negligible coprecipitation of high-density lipoprotein subfractions (HDL1, HDL2, HDL3), even in hypertriglyceridemic sera. In comparison with ultracentrifugation, the precipitation method yields, on the average, values that are 0.17 mmol/L lower for cholesterol values but almost identical for apolipoprotein A-I and phosphatidylcholine. Looking for delta 3,5-cholestadiene formed from cholesterol in the precipitation residue, we used "high-performance" liquid chromatography and "high-performance" thin-layer chromatography and found none.