Partial FI gene-independence of lambda-21 hybrid phages specifying chimeric terminases.
Partial FI gene-independence of lambda-21 hybrid phages specifying chimeric terminases.
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指定嵌合末端酶的 lambda-21 杂交噬菌体的部分 FI 基因独立性。
DOI：
10.1016/0042-6822(88)90090-6
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发表时间：
1988
期刊：
影响因子：
3.7
通讯作者：
Momany,T
中科院分区：
文献类型：
--
作者：
Feiss,M;Frackman,S;Momany,T
已经检查了FI基因在产生嵌合λ-21末端酶的一系列λ-21杂合噬菌体的生命周期中的作用。构建了杂交体的FI+和Fl-衍生物的同基因系列，并检查了噬菌体的生长特性。发现四种杂交体中的三种（杂交体51、67和33）能够在不存在FI基因产物（gpFI）的情况下形成噬菌斑并产生小爆发，但是三种噬菌体中的每一种在存在gpFI的情况下都健康得多。结论是三种嵌合末端酶均依赖于 gpFI。 FI-杂种比λFI-生长得更好的能力被推测是由于嵌合终止酶和λ终止酶之间的微小的定性或定量差异。第四个杂交体 (54)，从早期的工作中已知会产生弱终止酶，它比其他杂交体和 λ 本身更依赖于 gpFI。
The role of theFIgene in the life cycles of a series of λ-21 hybrid phages that produce chimeric λ-21 terminases has been examined. An isogenic series ofFI+andFl−derivatives of the hybrids was constructed, and the growth properties of the phages were examined. It was found that three of the four hybrids (hybrids 51, 67, and 33) are able to form plaques and produce a small burst in the absence of theFIgene product (gpFI), but each of the three phages is much healthier in the presence of gpFI. It is concluded that each of the three chimeric terminases is dependent on gpFI. The ability of theFI−hybrids to grow better than λFI−is postulated to be due to a minor qualitative or quantitative difference between the chimeric terminases and λ terminase. The fourth hybrid (54), known from earlier work to produce an infirm terminase, is more dependent on gpFI than the other hybrids and λ itself.