Novel bioactive nanocomposite for Class-V restorations to inhibit periodontitis-related pathogens.
Novel bioactive nanocomposite for Class-V restorations to inhibit periodontitis-related pathogens.
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DOI：
10.1016/j.dental.2016.09.023
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发表时间：
2016-12
期刊：
影响因子：
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通讯作者：
Lin Wang;M. Melo;M. Weir;Xianju Xie;M. Reynolds;H. Xu
中科院分区：
文献类型：
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作者：
Lin Wang;M. Melo;M. Weir;Xianju Xie;M. Reynolds;H. Xu
目的随着世界人口的老龄化和老年人牙齿保留的增加，牙根龋的发生率也在增加。有龈下边缘的V类牙周炎很难清洁，经常导致牙周炎。本研究的目的是开发一种V类复合材料含有二甲基氨基十六烷基甲基丙烯酸酯（DMAHDM）和纳米无定形磷酸钙（NACP），并调查机械性能和6种牙周炎相关的生物films的抑制为第一时间.MethodsEthoxylated双酚A二甲基丙烯酸酯（EBPADMA）和均苯四甲酸甘油二甲基丙烯酸酯（PMGDM）以1：1的质量比混合，形成树脂基质。DMAHDM、NACP和玻璃颗粒分别以3质量%、20质量%和50质量%掺入。共检测了6个菌种：牙龈卟啉单胞菌、中间普雷沃菌、变黑普雷沃菌、伴放线菌聚集杆菌、核瘤梭杆菌和粪肠球菌。结果在复合材料中加入3%的DMAHDM对复合材料的弯曲强度和弹性模量无明显影响（p > 0.1）。对于所有六种牙周病原体，DMAHDM复合物的生物膜CFU比没有DMAHDM的生物膜CFU低近三个数量级。DMAHDM复合剂对6种蜘蛛的杀灭效果为：E. faecalis<F. nucleatum <P. nigrescens= P. intermedia<A.放线菌共生菌<牙龈卟啉单胞菌。DMAHDM可显著降低牙周膜生物量和多糖含量（p < 0.05）。这种复合材料是有希望的V类牙周病，以恢复根龋和防治牙周炎。
ObjectivesThe occurrence of tooth root caries is increasing as the world population ages and tooth retention in seniors increases. Class V restorations with subgingival margins are difficult to clean and often lead to periodontitis. The objectives of this study were to develop a Class V composite containing dimethylaminohexadecyl methacrylate (DMAHDM) and nanoparticles of amorphous calcium phosphate (NACP), and investigate mechanical properties and the inhibition of six species of periodontitis-related biofilms for the first time.MethodsEthoxylated bisphenol A dimethacrylate (EBPADMA) and pyromellitic glycerol dimethacrylate (PMGDM) were mixed at 1:1 mass ratio to form the resin matrix. DMAHDM, NACP, and glass particles were incorporated at 3%, 20% and 50% by mass, respectively. Six species were tested:Porphyromonas gingivalis,Prevotella intermedia,Prevotella nigrescens,Aggregatibacter actinomycetemcomitans,Fusobacterium nucleatumandEnterococcus faecalis. Colony-forming units (CFU), live/dead assay, biomass via crystal violet staining, and polysaccharide production by biofilms were determined on composites.ResultAdding 3% DMAHDM to composite did not affect the flexure strength and elastic modulus (p > 0.1). For all six species of periodontal pathogens, the DMAHDM composite had biofilm CFU nearly three orders of magnitude less than that without DMAHDM. The killing efficacy of DMAHDM composite against the six species was:E. faecalis<F. nucleatum < P. nigrescens=P. intermedia<A. actinomycetemcomitans<P. gingivalis. Biofilm biomass and polysaccharide were also greatly reduced via DMAHDM (p < 0.05).SignificanceThe novel nanocomposite containing DMAHDM and NACP showed strong inhibiting effect against all six species of periodontitis-related pathogens. This composite is promising for Class V restorations to restore root caries and combat periodontitis.