Manganese and iron deficiency in Southern Ocean Phaeocystis antarctica populations revealed through taxon-specific protein indicators
Manganese and iron deficiency in Southern Ocean Phaeocystis antarctica populations revealed through taxon-specific protein indicators
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DOI：
10.1038/s41467-019-11426-z
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发表时间：
2019-08
影响因子：
16.6
通讯作者：
Miao Wu;Miao Wu;J. McCain;Elden Rowland;R. Middag;M. Sandgren;Andrew E. Allen;Andrew E. Allen;Erin M. Bertrand
中科院分区：
文献类型：
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作者：
Miao Wu;Miao Wu;J. McCain;Elden Rowland;R. Middag;M. Sandgren;Andrew E. Allen;Andrew E. Allen;Erin M. Bertrand
铁和光被认为是控制南大洋浮游植物生长的限制因素。然而，最近的实地证据表明，锰的可用性也可能发挥作用。在这里，我们研究了铁和锰对蛋白质表达和生理inPhaeocystis anaerotica，一个关键的南极初级生产者的影响。我们提供分类群特异性蛋白质组学证据表明，在原位南大洋Phaeocystipopulations经常经历的压力，由于结合低锰和铁的可用性。在培养条件下，低铁锰复合胁迫诱导棕囊藻蛋白质组发生大规模变化，导致光合机构重组。NaturalPhaeocystisopulations产生的蛋白质签名表明晚季锰和铁的压力，与同时观察到的刺激叶绿素生产后，添加锰或铁。这些结果表明锰是南大洋生产力的重要驱动因素，并证明了肽质谱法用于确定不完全宏量营养素消耗驱动因素的实用性。
Iron and light are recognized as limiting factors controlling Southern Ocean phytoplankton growth. Recent field-based evidence suggests, however, that manganese availability may also play a role. Here we examine the influence of iron and manganese on protein expression and physiology inPhaeocystis antarctica, a key Antarctic primary producer. We provide taxon-specific proteomic evidence to show that in-situ Southern OceanPhaeocystispopulations regularly experience stress due to combined low manganese and iron availability. In culture, combined low iron and manganese induce large-scale changes in thePhaeocystisproteome and result in reorganization of the photosynthetic apparatus. NaturalPhaeocystispopulations produce protein signatures indicating late-season manganese and iron stress, consistent with concurrently observed stimulation of chlorophyll production upon additions of manganese or iron. These results implicate manganese as an important driver of Southern Ocean productivity and demonstrate the utility of peptide mass spectrometry for identifying drivers of incomplete macronutrient consumption.