Positive selection for loss-of-function tat mutations identifies critical residues required for TatA activity
Positive selection for loss-of-function tat mutations identifies critical residues required for TatA activity
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DOI：
10.1128/jb.187.8.2920-2925.2005
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发表时间：
2005-04-01
影响因子：
3.2
通讯作者：
Palmer, T
中科院分区：
文献类型：
--
作者：
Hicks, MG;Lee, PA;Palmer, T
达特系统存在于许多细菌的细胞质膜中，是折叠蛋白质的一般输出途径。在这里，我们描述了一种方法的发展，氯霉素乙酰转移酶的运输的基础上，允许积极选择突变体缺陷的达特功能。我们已经证明了这种方法的实用性，通过选择新的功能丧失等位基因的tatA从池的随机taA突变。大多数被分离的突变落在TatA的两亲性区域，强调了这部分蛋白在TatA功能中发挥的关键作用。
The Tat system, found in the cytoplasmic membrane of many bacteria, is a general export pathway for folded proteins. Here we describe the development of a method, based on the transport of chloramphenicol acetyltransferase, that allows positive selection of mutants defective in Tat function. We have demonstrated the utility of this method by selecting novel loss-of-function alleles of tatA from a pool of random taA mutations. Most of the mutations that were isolated fall in the amphipathic region of TatA, emphasizing the pivotal role that this part of the protein plays in TatA function.