Nature of the effector cells responsible for antibody-dependent cell-mediated killing of Cryptococcus neoformans
Nature of the effector cells responsible for antibody-dependent cell-mediated killing of Cryptococcus neoformans
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负责抗体依赖性细胞介导的新型隐球菌杀伤的效应细胞的性质
DOI：
10.1128/iai.14.3.716-720.1976
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发表时间：
1976
影响因子：
3.1
通讯作者：
A. Allison
中科院分区：
文献类型：
--
作者：
R. Diamond;A. Allison
研究了在体外存在抗隐球菌抗体的情况下，能够杀死新生隐球菌的人外周血白细胞的类型。在37℃下与兔抗隐球菌抗体孵育4h后，原始隐球菌苗在混合单核细胞制剂(约30%单核细胞)中存活了24.1+/-2.7%(四个实验的平均+/-标准差)。去除吞噬细胞后，6个实验的存活率为36.4+/-4.6%，而在存在纯化的粒细胞时存活率为52.8%(两个实验的平均值)，在存在纯化的T细胞的情况下存活率为96.9+/-1%。在含有白细胞和正常兔血清的对照混合物中，或在含有抗隐球菌抗体而不含效应性白细胞的混合物中，没有任何显着的杀灭作用。效靶比低至6.25：1，具有显著的抗体依赖性杀菌活性。这些观察结果表明，除T细胞外，多种类型的外周血白细胞都具有抗体依赖性杀菌活性。
Studies were performed to identify the types of human peripheral blood leukocytes capable of killing Cryptococcus neoformans in the presence of anticryptococcal antibody in vitro. A total of 24.1 +/- 2.7% (mean +/- standard error of the mean of four experiments) of the original cryptococcal inoculum survived in a mixed mononuclear, cell preparation (approximately 30% monocytes) after 4 h of incubation at 37 degrees C with rabbit anticryptococcal antibody. When phagocytic cells were removed, there was 36.4 +/- 4.6% survival in six experiments, compared with 52.8% survival in the presence of purified granulocytes (mean of two experiments) and 96.9 +/- 1% survival in the presence of purified T cells. There was never any significant killing in control mixtures that contained leukocytes with normal rabbit serum nor in those that contained anticryptococcal antibody without effector leukocytes. Significant antibody-dependent fungicidal activity was seen with ratios of effector to target cells as low as 6.25:1. These observations indicate that multiple types of peripheral blood leukocytes, excluding T cells, are capable of antibody-dependent fungicidal activity.