THE EXPRESSION OF PTEN IN THE DEVELOPMENT OF MOUSE COCHLEAR LATERAL WALL
THE EXPRESSION OF PTEN IN THE DEVELOPMENT OF MOUSE COCHLEAR LATERAL WALL
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DOI：
10.1016/j.neuroscience.2013.11.017
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发表时间：
2014-01-31
期刊：
影响因子：
3.3
通讯作者：
Tokuda, M.
中科院分区：
文献类型：
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作者：
Dong, Y.;Sui, L.;Tokuda, M.
10 号染色体上磷酸酶和张力蛋白同源物缺失 (PTEN) 是一种肿瘤抑制基因，可调节多种细胞过程，包括增殖、生长、突触发生、神经和神经胶质瘤干/祖细胞更新。此外，PTEN还可以调节哺乳动物耳蜗中感觉细胞的增殖和毛束的分化。在本研究中，我们使用免疫荧光、蛋白质印迹和逆转录聚合酶链反应 (RT-PCR) 来揭示发育中的耳蜗侧壁中 PTEN 的表达，这对于调节 K+ 稳态至关重要。相对较高水平的 PTEN 最初在胚胎第 17.5 天 (E) 开始分化时在侧壁边缘细胞 (MC) 中表达。同样高水平的表达随后在出生后第 3 天 (P) 的分化根细胞 (RC) 中表达，然后在第 10 天在螺旋韧带纤维细胞 (SLF) 中表达。在成熟的耳蜗中，PTEN 表达在 MC 和 SLF 中较低或检测不到，但在 RC 及其过程中仍然很高。发育中的侧壁中 PTEN 的表达模式表明，它在调节耳蜗 K+ 稳态的细胞途径的分化中发挥着关键作用。 (C) 2013 国际广播组织。由爱思唯尔有限公司出版。保留所有权利。
Phosphatase and tensin homolog deleted on chromosome 10 (PTEN) is a tumor suppressor gene that regulates various cell processes including proliferation, growth, synaptogenesis, neural and glioma stem/progenitor cell renewal. In addition, PTEN can regulate sensory cell proliferation and differentiation of hair bundles in the mammalian cochlea. In this study we use immunofluorescence, Western blot and reverse transcriptase-polymerase chain reaction (RT-PCR) to reveal the expression of PTEN in the developing cochlear lateral wall, which is crucial for regulating K+ homeostasis. Relatively high levels of PTEN are initially expressed in the marginal cells (MCs) of the lateral wall at embryonic day (E) 17.5 when they start to differentiate. Similarly high levels are subsequently expressed in differentiating root cells (RCs) at postnatal day (P) 3 and then in spiral ligament fibrocytes (SLFs) at P 10. In the mature cochlea, PTEN expression is low or undetectable in MCs and SLFs but it remains high in RCs and their processes. The expression pattern for PTEN in the developing lateral wall suggests that it plays a critical role in the differentiation of the cellular pathways that regulate K+ homeostasis in the cochlea. (C) 2013 IBRO. Published by Elsevier Ltd. All rights reserved.