SERS aptatags: New responsive metallic nanostructures for heterogeneous protein detection by surface enhanced Raman spectroscopy
SERS aptatags: New responsive metallic nanostructures for heterogeneous protein detection by surface enhanced Raman spectroscopy
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DOI：
10.1002/adfm.200800301
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发表时间：
2008-09-10
影响因子：
19
通讯作者：
Bazan, Guillermo C.
中科院分区：
文献类型：
--
作者：
Fabris, Laura;Dante, Mark;Bazan, Guillermo C.
我们在这里报告的“适体标签”，其中包括适体修饰的银纳米粒子（NP）举行在一起的光学记者。有可能使用这些材料来设计用于蛋白质鉴定的异质方法，该方法利用金属纳米结构的拉曼信号增强和适体的识别能力。通过将银NP与有机二硫醇分子连接，然后用对应于适体探针序列的硫醇化单链DNA（ssDNA）进行表面修饰来形成适体标签。传感表面包括含有硫醇化捕获适体和巯基十六烷酸的银层，以使非特异性结合最小化。整个过程提供了出色的选择性和灵敏度。通过Sers图和原子力显微镜对传感表面进行了详细表征，以了解结构特征如何导致信号产生。
We report here "aptatags," which consist of aptamer-modified silver nanoparticles (NPs) held together by an optical reporter. It is possible to use these materials to design a heterogeneous method for protein identification that takes advantage of the Raman signal enhancement by metallic nanostructures and the recognition capabilities of aptamers. Aptatags are formed by linking silver NPs with an organic dithiol molecule, followed by surface modification with thiolated single-stranded DNA (ssDNA) corresponding to the sequence of the aptamer probe. The sensing surface involves a silver layer containing the thiolated capturing aptamer and mercaptohexadecanoic acid to minimize nonspecific binding. The overall process provides excellent selectivity and sensitivity. Detailed characterization of the sensing surface by SERS maps and atomic force microscopy was carried out to understand how structural features lead to signal generation.