Effects of hormone treatments and temperature on sex-reversal of Nigorobuna Carassius carassius grandoculis
Effects of hormone treatments and temperature on sex-reversal of Nigorobuna Carassius carassius grandoculis
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DOI：
10.1046/j.1444-2906.2002.00507.x
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发表时间：
2002-08-01
影响因子：
1.9
通讯作者：
Fujioka, Y
中科院分区：
文献类型：
--
作者：
Fujioka, Y
在发育早期，采用17-甲基睾酮（17-MT）浸泡和控制水温的方法，研究了全雌鳙鱼（nigorobuna Carassius grandoculis）向表型雄性的功能性性别逆转。从鱼苗孵化后第20天开始，在24度和30度不同浓度的17-MT水中饲养80天。虽然在24度温度下暴露于0.1和1.0马克/升17-MT的鱼都是雄性，但10.0马克/升17-MT的鱼有43%是雌性。30°c处理的雄虫占22%，24°c处理的雄虫全部为雌虫，30°c处理的雄虫比例为0.01 ~ 1.0 μ g/L，略低于24°c处理。这些结果表明，黑蛾的性别表型表达具有热稳定性，性别决定受遗传因素和温度的控制。此外，在早期发育过程中控制温度已被证明对生产全雌性后代很重要，这些后代可作为池塘养殖的种畜，用于制备“funazushi”。
Functional sex reversal of all-female nigorobuna Carassius carassius grandoculis to phenotypic males was examined by immersion exposure of fry to 17-methyltestosterone (17-MT) and controlled water temperature during early development. Fry were reared in water containing different concentrations of 17-MT at 24 and 30degreesC for 80 days starting 20 days after hatching. Although the fish exposed to 0.1 and 1.0 mug/L 17-MT at 24degreesC were all male, treatment with 10.0 mug/L 17-MT resulted in 43% females. Twenty-two percent males appeared in the control treatment at 30degreesC but the control at 24degreesC was entirely female, The proportion of males in treatments exposed to 0.01-1.0 mug/L 17-MT at 30degreesC was slightly lower than in the respective treatments at 24degreesC. These results indicate that the phenotypic expression of sex in nigorobuna is thermolabile and that sex determination is under the control of genetic factors and temperature. Also, control of temperature during early development has been shown to be important for the production of all-female offspring for use as breeding stock for pond culture of fish suitable for preparation as 'funazushi'.