CASEIN KINASE-II IS THE P-PROTEIN PHOSPHORYLATING CELLULAR KINASE ASSOCIATED WITH THE RIBONUCLEOPROTEIN COMPLEX OF PURIFIED VESICULAR STOMATITIS-VIRUS
CASEIN KINASE-II IS THE P-PROTEIN PHOSPHORYLATING CELLULAR KINASE ASSOCIATED WITH THE RIBONUCLEOPROTEIN COMPLEX OF PURIFIED VESICULAR STOMATITIS-VIRUS
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DOI：
10.1099/0022-1317-76-2-365
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发表时间：
1995-02-01
影响因子：
3.8
通讯作者：
BANERJEE, AK
中科院分区：
文献类型：
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作者：
GUPTA, AK;DAS, T;BANERJEE, AK
从水疱性口炎病毒（VSV）病毒粒子中分离并鉴定了与高度纯化的转录活性核糖核蛋白复合物相关的蛋白激酶活性。根据几种生化和免疫学标准，磷酸化细菌表达的未磷酸化（P-o）蛋白的蛋白激酶活性被证明是细胞酪蛋白激酶II （CKII）。这些研究包括特异性抑制剂对蛋白激酶的抑制，突变磷酸化蛋白(P)的磷酸化，CKII抗体和Western blot分析的免疫沉淀，以及最终激活P的能力，从而在转录-重构反应中合成RNA。P蛋白在细胞内被细胞CKII磷酸化。目前的研究表明，VSV特异性包装CKII，在形态发生过程中，CKII仍然与核糖核蛋白复合物密切相关。
Protein kinase activities associated with a highly purified transcriptionally active ribonucleoprotein complex from the virions of vesicular stomatitis virus (VSV) were isolated and characterized. Based upon several biochemical and immunological criteria, the protein kinase activity, which phosphorylated the bacterially expressed unphosphorylated (P-o) protein, was shown to be cellular casein kinase II (CKII). These studies included inhibition of the protein kinase by specific inhibitors, phosphorylation of mutant phosphoproteins (P), immunoprecipitation by CKII antibody and Western blot analyses, and finally its ability to activate P, to synthesize RNA in a transcription-reconstitution reaction. The P protein is phosphorylated intracellularly by cellular CKII. The present study demonstrates that VSV specifically packages CKII which remains strongly associated with the ribonucleoprotein complex during morphogenesis.