Alterations in BDNF and NT-3 mRNAs in rat hippocampus after experimental brain trauma
Alterations in BDNF and NT-3 mRNAs in rat hippocampus after experimental brain trauma
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DOI：
10.1016/s0169-328x(97)00158-7
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发表时间：
1997-09-01
期刊：
影响因子：
--
通讯作者：
Seroogy, KB
中科院分区：
文献类型：
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作者：
Hicks, RR;Numan, S;Seroogy, KB
先前的研究表明，神经营养蛋白脑源性神经营养因子（BDNF）和神经营养蛋白-3（NT-3）对于各种脑损伤后的海马神经元的某些亚群具有神经保护或神经营养作用。本研究检测了脑外伤后大鼠海马 BDNF 和 NT-3 mRNA 的表达。中度（2.0-2.1 atm）侧向液体冲击（FP）脑损伤或假损伤后，对成年大鼠的海马进行处理，使用 S-35 标记的 cRNA 对 BDNF 和 NT-3 mRNA 进行原位杂交定位。损伤后存活时间为 1、3、6、24 和 72 小时的探针。单侧 FP 损伤显着增加了双侧齿状回 BDNF mRNA 的杂交，在 3 小时达到峰值，并在损伤后长达 72 小时内保持高于对照水平。在 FP 损伤后 1、3 和 6 小时，在海马 CA3 区域双侧还观察到 BDNF mRNA 表达适度增加，但表达在 24 小时时下降至对照水平。相反，在 FP 损伤后 6 小时和 24 小时存活时间，齿状回中 NT-3 mRNA 显着减少。这些结果表明，FP 脑损伤差异性地调节海马中 BDNF 和 NT-3 mRNA 的表达，并表明神经营养素可塑性是海马神经元对脑损伤的功能反应。 (C) 1997 Elsevier Science B.V.
Previous studies have suggested that the neurotrophins brain-derived neurotrophic factor (BDNF) and neurotrophin-3 (NT-3) are neuroprotective or neurotrophic for certain subpopulations of hippocampal neurons following various brain insults. In the present study, the expression of BDNF and NT-3 mRNAs in rat hippocampus was examined after traumatic brain injury. Following lateral fluid percussion (FP) brain injury of moderate severity (2.0-2.1 atm) or sham injury, the hippocampi from adult rats were processed for the in situ hybridization localization of BDNF and NT-3 mRNAs using S-35-labeled cRNA. probes at post-injury survival times of 1, 3, 6, 24 and 72 h. Unilateral FP injury markedly increased hybridization for BDNF mRNA in the dentate gyrus bilaterally which peaked at 3 h and remained above control levels for up to 72 h post-injury. A moderate increase in BDNF mRNA expression was also observed bilaterally in the CA3 region of the hippocampus at 1, 3, and 6 h after FP injury, but expression declined to control levels by 24 h. Conversely, NT-3 mRNA was significantly decreased in the dentate gyrus following FP injury at the 6 and 24 h survival times. These results demonstrate that FP brain injury differentially modulates expression of BDNF and NT-3 mRNAs in the hippocampus, and suggest that neurotrophin plasticity is a functional response of hippocampal neurons to brain trauma. (C) 1997 Elsevier Science B.V.