Natural variation in the consequences of gene overexpression and its implications for evolutionary trajectories.
Natural variation in the consequences of gene overexpression and its implications for evolutionary trajectories.
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DOI：
10.7554/elife.70564
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发表时间：
2021-08-02
期刊：
影响因子：
7.7
通讯作者：
Gasch AP
中科院分区：
文献类型：
--
作者：
Robinson D;Place M;Hose J;Jochem A;Gasch AP
通过基因或染色体扩增的拷贝数变异为快速表型变异提供了途径，并支持基因功能的长期进化。虽然拷贝数变异的进化重要性是已知的，很少有人知道遗传背景如何影响其耐受性。在这里，我们测量了超过4000个过表达的基因在15个酿酒酵母菌株代表不同的血统，探索自然变异的耐受基因过表达（OE）的健身成本。菌株特异性的影响占主导地位的基因OE的健身成本。我们报告的全球差异基因OE的后果，独立的扩增基因，以及基因特异性的影响，依赖于遗传背景。对基因OE的反应的自然变异可以用几种模型来解释，包括菌株特异性生理差异、资源限制和调节敏感性。这项工作提供了新的见解遗传背景如何影响耐受基因扩增和进化轨迹访问不同的背景。
Copy number variation through gene or chromosome amplification provides a route for rapid phenotypic variation and supports the long-term evolution of gene functions. Although the evolutionary importance of copy-number variation is known, little is understood about how genetic background influences its tolerance. Here, we measured fitness costs of over 4000 overexpressed genes in 15 Saccharomyces cerevisiae strains representing different lineages, to explore natural variation in tolerating gene overexpression (OE). Strain-specific effects dominated the fitness costs of gene OE. We report global differences in the consequences of gene OE, independent of the amplified gene, as well as gene-specific effects that were dependent on the genetic background. Natural variation in the response to gene OE could be explained by several models, including strain-specific physiological differences, resource limitations, and regulatory sensitivities. This work provides new insight on how genetic background influences tolerance to gene amplification and the evolutionary trajectories accessible to different backgrounds.